| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2526SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47339SS | |||
| Azide and BSA Free | NBP2-80748 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-167SS | |||
| Azide and BSA Free | NBP2-80826 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27350 | |||
| BSA Free | NB100-56747SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56527SS | |||
| Azide and BSA Free | NBP2-80616 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22127SS | |||
| Azide and BSA Free | NBP2-80575 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-81601SS | |||
![Western Blot: HRD1 Antibody [NB100-2526] - Hrd1 is required for CDT intoxication. Co-immunoprecipitation of Derl2 and Hrd1. Derl2 was immunoprecipitated and samples were analyzed for Hrd1 by western blot. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004295) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HRD1-Antibody-Western-Blot-NB100-2526-img0006.jpg "Western Blot: HRD1 Antibody [NB100-2526] - Hrd1 is required for CDT intoxication. Co-immunoprecipitation of Derl2 and Hrd1. Derl2 was immunoprecipitated and samples were analyzed for Hrd1 by western blot. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004295) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: HRD1 Antibody [NB100-2526] - HRD1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2526-img0003.jpg)
![Immunohistochemistry-Paraffin: HRD1 Antibody [NB100-2526] - FFPE tissue section of human tonsil using rabbit polyclonal HRD1 antibody at 2 ug/mL concentration (1:500 dilution). The primary antibody binding to SYVN1/HRD1 antigen in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This HRD1 antibody generated an expected cytoplasmic staining of HRD1 with stronger signal around periphery of the nucleus signifying the staining of the endoplasmic reticulum.](http://images.novusbio.com/fullsize/HRD1-Antibody-Immunohistochemistry-Paraffin-NB100-2526-img0004.jpg)
![Western Blot: HRD1 Antibody [NB100-2526] - Total protein from human HeLa, Hek293 and mouse 3T3 cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HRD1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/HRD1-Antibody-Western-Blot-NB100-2526-img0005.jpg)
![Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Western-Blot-NBP1-47339-img0006.jpg "Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0004.jpg)
![Flow Cytometry: GAPDH Antibody (1A10) [NBP1-47339] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Flow-Cytometry-NBP1-47339-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunohistochemistry-Paraffin-NBP1-47339-img0005.jpg)
![Simple Western: GAPDH Antibody (1A10) [NBP1-47339] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Simple-Western-NBP1-47339-img0007.jpg)
![Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Western-Blot-NB200-167-img0003.jpg "Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)")
![Immunohistochemistry-Paraffin: LANA2 Antibody (CM-A807) [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource and performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA. Representative image shows the LANA staining of skin KS lesion. Image collected and cropped by CiteAb from the following publication (//mbio.asm.org/lookup/doi/10.1128/mBio.02217-17), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Immunohistochemistry-Paraffin-NB200-167-img0004.jpg)
![Immunohistochemistry: LANA2 Antibody (CM-A807) - BSA Free [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource & performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA & anti-vIRF3 (CM-A807). Representative images show the LANA staining of skin KS lesion & the vIRF3 staining of skin, tonsil, & mouth KS lesions & positive staining corresponding to either LANA or vIRF3 in total KS biopsy specimens embedded in TMA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29339432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-167_mouse-monoclonal-lana2-antibody-cm-a807-310202415371954.jpg)
![Western Blot: BID Antibody [NB100-56106] - Analysis of Bid in normal mouse tissues using this antibody. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).](http://images.novusbio.com/fullsize/BID-Antibody-Western-Blot-NB100-56106-img0008.jpg "Western Blot: BID Antibody [NB100-56106] - Analysis of Bid in normal mouse tissues using this antibody. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 kDa Bid and the ~15 kDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).")
![Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Two cores from a human ovarian carcinoma formalin-fixed, paraffin-embedded tissue microarray demonstrate the variable expression of Bid protein. The sections were stained with Bid antibody at 1:2000 with hematoxylin-eosin counterstain. Section A shows very weak staining while section B stains much more strongly.](http://images.novusbio.com/fullsize/BID-Antibody-Immunohistochemistry-Paraffin-NB100-56106-img0009.jpg)
![Western Blot: BID Antibody [NB100-56106] - Analysis of BID in NRK whole cell lysate using anti-BID antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/BID-Antibody-Western-Blot-NB100-56106-img0007.jpg)
![Immunohistochemistry-Paraffin: BID Antibody [NB100-56106] - Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using this antibody at 1:2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.](http://images.novusbio.com/fullsize/BID-Antibody-Immunohistochemistry-Paraffin-NB100-56106-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-19775-img0004.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Analysis of IL1 beta Antibody in extracts from HeLa cells.")
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody - BSA Free [NBP1-19775] - In situ expression of IL-6 in glomerular and tubulointerstitial compartments in patients with diabetic nephropathy DN (right) and control group (left). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32723296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody---BSA-Free-Immunohistochemistry-NBP1-19775-img0006.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-19775] - Staining in the cytoplasm of villi and in the crypt cells of human lung carcinoma tissue.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-19775-img0005.jpg)
![Western Blot: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Analysis of DR4 in Daudi cell lysate using DR4 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TRAIL-R1-TNFRSF10A-Antibody-32A1380-Western-Blot-NB100-56747-img0002.jpg "Western Blot: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Analysis of DR4 in Daudi cell lysate using DR4 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Simple Western lane view shows a specific band for TRAIL R1 in 0.5 mg/ml of Daudi lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/TRAIL-R1-TNFRSF10A-Antibody-32A1380-Simple-Western-NB100-56747-img0003.jpg)
![Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0018.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Caspase-8 expression in 786-O and A-498 cellsClose](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0022.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) [NB100-56527] - Formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) stained with Caspase-8 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Cancer/normal adjacent tissue array was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Immunohistochemistry-Paraffin-NB100-56527-img0014.jpg)
![Flow Cytometry: Caspase-8 Antibody (90A992) [NB100-56527] - Flow cytometric analysis of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody was used for this test. IC-Flow (Intracellular Staining Flow Cytometry Kit) was used to fix and prepare the cells for staining.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Flow-Cytometry-NB100-56527-img0008.jpg)
![Western Blot: Caspase-8 Antibody (90A992) [NB100-56527] - Analysis using the Biotin conjugate of NB100-56527. Detection of human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Western-Blot-NB100-56527-img0021.jpg)
![Simple Western: Caspase-8 Antibody (90A992) [NB100-56527] - Simple Western lane view shows a specific band for Caspase 8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-90A992-Simple-Western-NB100-56527-img0019.jpg)
![Simple Western: Mouse Monoclonal Caspase-8 Antibody (90A992) [NB100-56527] - S2/IAPinh induces activation of the extrinsic apoptosis pathway via degradation of cIAP-1. (A) Schematic diagram of the intrinsic and extrinsic apoptosis pathways: arrows represent stimulation. TNFR, tumor necrosis factor receptor; DR4-5, death receptor 4–5; cIAP-1/2, cellular inhibitor of apoptosis protein-1/2; RIPK1, Receptor-interacting serine/threonine-protein kinase 1. (B) Protein expression of cIAP-1, cIAP-2, and XIAP in HPAC cells treated with vehicle, SW43 (10 µM), IAPinh (10 µM), or S2/IAPinh (10 µM) for 6 h. The precursor and cleaved forms of caspases 3, 8, and 9 were also analyzed for these cells using Wes automated capillary blotting system (Protein Simple). (C) Quantification of protein expression. Relative densitometry of each band normalized to the total protein. Data shown as means ± SEM. **P Sci Rep (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56527_mouse-monoclonal-caspase-8-antibody-90a992-12220251513481.jpg)
![Simple Western: Mouse Monoclonal Caspase-8 Antibody (90A992) [NB100-56527] - S2/IAPinh induces tumor cell death via activation of the extrinsic apoptosis pathway in vivo. KP2 tumor samples collected from animals at 48 h after the treatment with either vehicle, SW43, IAPinh, or S2/IAPinh, were used for analyses. (A) Representative images of TUNEL labeled apoptosis cells in KP2 tumor samples of each group. Nuclei were stained in blue with Hoechst, TUNEL positive cells are in red. Scale bars are equal to 20 µm. (B) Quantification of TUNEL positive cells per area in each group. Data are shown as means ± SEM; ****P Sci Rep (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56527_mouse-monoclonal-caspase-8-antibody-90a992-12220251513480.jpg)
![Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Western-Blot-NBP2-16521-img0021.jpg "Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.")
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Mouse duodenum. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0028.jpg)
![<b>Genetic Strategies Validation. </b>Chromatin Immunoprecipitation (ChIP): FOXO3 Antibody [NBP2-16521] - (D) ChIP assay for LMOD1 and EGFR (as a positive control region) in HCASMC chromatin lysates immunoprecipitated with antibodies to FOXO3 or a negative control rabbit IgG. n = 3. (E) Allele specific ChIP (haploChIP) for FOXO3 protein in DNA derived from cultured HCASMC ChIP experiments. DNA from cell line homozygous for the ancestral allele was used as a positive control and arbitrarily set to 1. Values represent mean +/- standard deviation of triplicates. Similar results were observed from n = 4 independent lines for each genotype. (F) Quantitative RT-PCR analysis of LMOD1 in HCASMCs following knock down of endogenous FOXO3 (F) or overexpression of FOXO3 (G). The results were reproduced in n = 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007755) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16521-img0030.jpg)
![Western Blot: FOXO3 Antibody [NBP2-16521] - 50 ug of mouse brain whole cell lysate). Antibody at 1:500.](http://images.novusbio.com/fullsize/FOXO3A-Antibody-Western-Blot-NBP2-16521-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Huh-7 xenograft, using FOXO3A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/FOXO3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Rat colon. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0024.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Rat brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0026.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Mouse brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunohistochemistry-Paraffin-Negative-NBP2-16521-img0027.jpg)
![Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - Jurkat whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 ug of FOXO3A antibody 5% SDS-PAGE The immunoprecipitated FOXO3A protein was detected by FOXO3A antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/FOXO3-Antibody-Immunoprecipitation-NBP2-16521-img0029.jpg)
![Western Blot: FOXO3 Antibody [NBP2-16521] - LMOD1 expression is mediated by PDGF-BB-FOXO3 signaling cascade.(A) UCSC Browser screenshot of the LMOD1 CAD locus at chromosome 1q32.1 highlighting the candidate causal variant, rs34091558, overlapping ATAC-seq open chromatin & RNA-seq tracks in HCASMCs treated with PDGF-BB (n = 2 biological replicates). Genomic coordinates refer to hg19 assembly. Quantitative RT-PCR (B) & Western blotting (C) data revealing PDGF-BB & phosphorylated-FOXO3 (P-FOXO3) mediated LMOD1 expression. (D) Co-expression microarray analysis performed in a cohort of carotid atherosclerotic plaques (n = 127) indicating that LMOD1 & FOXO3 are positively correlated in arterial tissues. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30444878), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-310202415345315.jpg)
![Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - Chemotherapy-induced A2BR expression promotes FOXO3 binding on pluripotency factor genes through decreased H3K27me3 & increased H3K27ac chromatin marks. A & B, MDA-MB-231 NTC or A2BR knockdown subclones were transfected with pLX304 (empty vector, EV) or pLX304 encoding A2BR. Cells were treated with vehicle (V) or 10 nM paclitaxel (P) for 72 h & chromatin immunoprecipitation (ChIP) was performed with antibody against FOXO3. Primers flanking the FOXO3 binding site in the NANOG, SOX2 & KLF4 gene (A) were used for qPCR (B; mean ± SEM; n = 3); **p Sample: Paraffin-embedded human glioblastoma.FOXO3A stained by FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-immunohistochemistry-paraffin-2611202518191319.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-western-blot-2611202518303821.jpg)
![FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXO3A stained by FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-immunocytochemistry-immunofluorescence-261120251845408.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-western-blot-261120251824390.jpg)
![FOXO3A antibody [C3], C-term detects FOXO3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.FOXO3A stained by FOXO3A antibody [C3], C-term (NBP2-16521) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16521_rabbit-polyclonal-foxo3-antibody-immunohistochemistry-paraffin-2611202518441020.jpg)
![Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Simple-Western-NBP2-22127-img0006.jpg "Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunocytochemistry-Immunofluorescence-NBP2-22127-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Ability to combat ROS varies across cell lines. Western blot analysis showing levels of AMPK and activated AMPK (T172) at 24, 48 and 72 hours following exposure to 0.4 uM GNE-617. Full-length western blots are shown in S2D Fig. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0164166), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Western-Blot-NBP2-22127-img0009.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Immunohistochemistry-Paraffin-NBP2-22127-img0005.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on NIH3T3 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0008.jpg)
![Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-Flow-Cytometry-NBP2-22127-img0007.jpg)
![ELISA: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/AMPK-alpha-1-Antibody-2B7-ELISA-NBP2-22127-img0001.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0012.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.")
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Staining of mouse carotid artery. A) No Ab staining of WT uninjured Ms. B) IL-1 beta staining of cells after surgical injury of tissue. C) No Ab staining of injured tissue from an IL-1 beta KO.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42767-img0004.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45C RT](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-42767-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0008.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0011.jpg)
![Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Western-Blot-NB120-6401-img0011.jpg "Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Human pancreas cancer cells. Antibody at 1:250 dilution. Overnight incubation. No permeabilization. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunocytochemistry-Immunofluorescence-NB120-6401-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Staining of FFPE human placenta sections with 1:300 Monoclonal Anti-Cytokeratin, pan Clone: PCK-26, followed by Goat Anti Mouse IgG (Fab)-FITC.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Immunohistochemistry-Paraffin-NB120-6401-img0006.jpg)
![Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Histological examination of the subcutaneous xenografts in nude mice with H&E staining (left panel) and IHC with anti-pan CK (right panel) at 400x magnification. Asterisks (*) indicate representative keratinization and keratin-pearl formation. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6694/12/1/61), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0014.jpg)
![Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Histological examination of NHRI-HN1 tumors in nude mice and B6 mice with H&E staining (upper panels) at 400x and 1000x magnifications and IHC with anti-pan CK (middle panel) and EGFR (lower panel) at 400x magnification. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6694/12/1/61), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Detection of EMT & increased motility in NHRI-HN1 cells. (A) Summary of the most enriched pathways associated with tumorigenesis in syngeneic mice by comparing tumorigenic NHRI-HN1 cells with nontumorigenic cells, including M1-2, M2-3 & NHRI-HN2, using GSEA analysis. Blue indicates a negative normalized enrichment score (NES) & orange indicates a positive NES. (B) Morphology of M1-2 & NHRI-HN1 cells via phase-contrast microscopy at 100× magnification. (C) Relative adhesion activity in M1-2 & NHRI-HN1 cells, determined by normalizing the mean OD 490 nm value of NHRI-HN1 cells to that of M1-2 cells. (D) Cells stained with Alexa Fluor 488 phalloidin, anti-pan-CK & anti-EGFR antibodies at 400× magnification. (E) Immunoblot analysis of epithelial (E-cadherin, alpha -catenin & beta -catenin), mesenchymal (N-cadherin & Vimentin) proteins & EMT-related transcription factors, including Twist, Snail & Slug in M1-2 & NHRI-HN1 cells. Protein levels were normalized to an internal control, alpha -tubulin. Ratios were determined by dividing the normalized protein levels in NHRI-HN1 cells by that in M1-2 cells. (F) Representative images (left) & relative data (right) for migration activity of M1-2 & NHRI-HN1 cells. (G) Representative images (left) & relative data (right) for invasion activity of M1-2 & NHRI-HN1 cells at 200× magnification. The relative migration or invasion activity was determined by normalizing the mean number of cells that have migrated or invaded per field of NHRI-HN1 cells to that of M1-2 cells. Error bars represent SE; ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31878324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-6401_mouse-monoclonal-cytokeratin-pan-antibody-pck-26-310202416212383.jpg)
![Western Blot: TRPM2 Antibody [NB110-81601] - Lane 1: CHO cells untransfected. Lane 2: CHO cell transfected with rat TRPM2 Lane 3: Mouse cortical neurons (cultured) Photo courtesy of Dr. Paco Herson, Oregon Health & Science University.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Western-Blot-NB110-81601-img0007.jpg "Western Blot: TRPM2 Antibody [NB110-81601] - Lane 1: CHO cells untransfected. Lane 2: CHO cell transfected with rat TRPM2 Lane 3: Mouse cortical neurons (cultured) Photo courtesy of Dr. Paco Herson, Oregon Health & Science University.")
![Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TRPM2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-81601-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Staining of rat hippocampus using NB110-81601. TRPM2 is stained red, GFAP is stained green and nuclei staining is blue. Photo courtesy of Dr. Ji-Zhong Bai, The University of Auckland, New Zealand.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-81601-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - TRPM2 antibody was tested in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-81601-img0009.jpg)