Gpi Anchoring: PTMs Bioinformatics

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Gpi Anchoring Bioinformatics

Glycosylphosphatidylinositol (GPI) is a glycolipid that is covalently attached to the C-terminus during a process called glypiation. Glypiation takes place in the ER, beginning on the cytoplasmic side and ending on the luminal side. GPI anchoring results in the recruitment of proteins to cell membranes. Following their covalent attachment, GPI anchors may also be cleaved by enzymes like phospholipase C.

Gpi Anchoring Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Gpi Anchoring below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1079 products for the study of Gpi Anchoring that can be applied to Western Blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Immunohistochemistry from our catalog of antibodies and ELISA kits.

NBP2-02541
Western Blot: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] Analysis of extracts (35ug) from 9 different cell lines by using anti-Glucose 6 phosphate isomerasemonoclonal antibody.Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (2D2) [NBP2-02541] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Glucose 6 phosphate isomerase.

Mouse Monoclonal
Species Human, Primate
Applications WB, ICC/IF, IHC

NBP2-24557
Western Blot: GNPDA1 Antibody [NBP2-24557] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 25 ug/ml.Immunohistochemistry-Paraffin: GNPDA1 Antibody [NBP2-24557] - Formalin-fixed paraffin-embedded human kidney tissue stained with GNPDA1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Rabbit Polyclonal
Species Human, Equine, Primate
Applications WB, IHC-P

NB100-56605
Western Blot: Prion protein Antibody [NB100-56605] - Analysis of BSE in (A) recombinant fusion protein containing amino acids 162-170 and (B) fusion partner without these amino acids, using this antibody at 5 ug/ml.

Rabbit Polyclonal
Species Avian, Bovine, Sheep
Applications WB

NBP2-13760
Immunohistochemistry-Paraffin: PIGA Antibody [NBP2-13760] - Staining of human gallbladder shows granulated cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

AF2009
CD55/DAF was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 15 µg/mL Goat Anti-Human CD55/DAF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2009) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, IHC

3 Publications
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

13 Publications
NBP1-84550
Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker  [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10.  Lane 2: Human cell line Daudi.Immunocytochemistry/Immunofluorescence: HLA DQA1 Antibody [NBP1-84550] - Staining of human cell line U-251MG shows positivity in cytoplasm.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-88263
Western Blot: C4 binding protein A Antibody [NBP1-88263] - Analysis in human cell line RT-4, human cell line U-251 MG, human cell line A-431, human liver tissue and human tonsil tissue.Immunohistochemistry-Paraffin: C4 binding protein A Antibody [NBP1-88263] - Staining of human small intestine shows distinct positivity in plasma.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

H00007001-M01
Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 monoclonal antibody (M01), clone 4E10-2D2 Analysis of PRDX2 expression in Hela.Immunocytochemistry/Immunofluorescence: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Analysis of monoclonal antibody to PRDX2 on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

     1 Review

4 Publications
NBP1-91642
Immunocytochemistry/Immunofluorescence: ABCB6 Antibody [NBP1-91642] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus.Immunohistochemistry-Paraffin: ABCB6 Antibody [NBP1-91642] - Staining of human testis shows cytoplasmic positivity.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

1 Publication
H00079092-T01
Lane 1: CARD14 transfected lysate (47.85 KDa) Lane 2: Non-transfected lysate.CARD14 transfected lysate.


Species Human

NB500-330
Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.

Mouse Monoclonal
Species Human
Applications B/N, Flow, IHC

5 Publications
NBP1-85740
Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Analysis in human cell line U-87 MG.Immunocytochemistry/Immunofluorescence: 5'-Nucleotidase/CD73 Antibody [NBP1-85740] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Rabbit Polyclonal
Species Human, Porcine
Applications WB, ICC/IF, IHC

     4 Reviews

5 Publications
NBP1-84796
Western Blot: ERp57/PDIA3 Antibody [NBP1-84796] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP1-84796] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

4 Publications
AF5128
Western blot shows lysates of DU145 human prostate carcinoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5128) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB

AF4466
Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC‑ beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse PLC‑ beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Sheep Polyclonal
Species Human, Mouse
Applications WB

NBP2-52532
Western Blot: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] -  Analysis using PLCG1 mouse mAb against Hela (1), A431 (2), C6 (3), NIH/3T3 (4), COS7 (5), and HCT116 (6) cell lysate.Immunohistochemistry-Paraffin: PLC-gamma 1 Antibody (3C2D11) [NBP2-52532] - Analysis of paraffin-embedded bladder cancer tissues using PLCG1 mouse mAb with DAB staining.

Mouse Monoclonal
Species Human, Rat, Monkey
Applications WB, ELISA, Flow

MAB6240
Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB6240) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB6240) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human
Applications WB, IHC