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Acylation Bioinformatics

Acylation is a type of post-translational modification (PTM) that involves the attachment of an acyl group to a protein's amino acid residues. Proteins may be acylated by covalently bonding various fatty acids. Acylation has an impact on protein function and intracellular localization.

Acylation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Acylation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 2175 products for the study of Acylation that can be applied to Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB200-540
Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.

Rat Monoclonal
Species Mouse, E. coli
Applications Flow, IA, ICC/IF

     2 Reviews

18 Publications
NBP1-31682
Western Blot: NDUFAB1 Antibody [NBP1-31682] - 30 ug PC-12 whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:5000.Immunocytochemistry/Immunofluorescence: NDUFAB1 Antibody [NBP1-31682] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-31754
Western Blot: ASPA Antibody [NBP1-31754] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody [N1C3-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: ASPA Antibody [NBP1-31754] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aspartoacylase protein stained by  partoacylase antibody [N1C3-2] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody  diluted at 1:1000. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Monkey
Applications WB, ICC/IF, IHC

NB110-53818
Western Blot: ATG5 Antibody [NB110-53818] - Western blot analysis in mouse wildtype ES cell lysate (Lane 1) using NB110-53818. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.Western Blot: ATG5 Antibody [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms3799), licensed under a CC-BY licence.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, EM

     7 Reviews

238 Publications
NBP1-90081
Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Analysis in human testis and prostate tissues using NBP1-90081 antibody. Corresponding ROPN1L RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human bronchus shows strong membranous positivity in respiratory epithelial cells.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP1-90271
Western Blot: ACF Antibody [NBP1-90271] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG spImmunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human colon shows moderate nuclear/cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP1-90927
Western Blot: gamma-glutamyl hydrolase Antibody [NBP1-90927] - Analysis in control (vector only transfected HEK293T lysate) and GGH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: gamma-glutamyl hydrolase Antibody [NBP1-90927] - Staining of human skeletal muscle shows very weak cytoplsmic positivity in myocytes.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP2-16655
Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term  diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: Ghrelin Receptor/GHSR Antibody [NBP2-16655] -  CL1-0 xenograft, using GHS-R1 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

1 Publication
NBP2-24653
Western Blot: GNPDA1 Antibody [NBP2-24653] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen and 4) rat spleen using GNPDA1 antibody at 1.0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.Immunohistochemistry-Paraffin: GNPDA1 Antibody [NBP2-24653] - Formalin-fixed paraffin-embedded human kidney tissue stained with GNPDA1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

AF1067
    Western  blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF  membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    Recombinant  Human Growth Hormone protein was serially diluted 2-fold and captured by  Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

     3 Reviews

9 Publications
MAB1417
Insulin was detected in immersion fixed  beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

14 Publications
MAB1455
Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=    Simple  Western lane view shows lysates of human serum, loaded at  1:25000. A specific band was detected for Albumin at  approximately 64 kDa (as indicated) using 1 µg/mL of Mouse  Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) .  This experiment was conducted under reducing conditions and using  the 12-230 kDa separation system.

Mouse Monoclonal
Species Human
Applications WB, Simple Western, IHC

     5 Reviews

30 Publications
210-TA
1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     36 Reviews

708 Publications
9094-AG
Recombinant Human ASIP/Agouti (Catalog # 9094-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED<sub>50</sub> for this effect is 0.8-4 μg/mL.


Species Human
Applications BA

NB100-1519
Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining (0.3ug/ml) of Jurkat (A) and (0.5ug/ml) HepG2 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

     1 Review

1 Publication
NB100-2278
Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells . Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250.  Detection:  Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.

Rabbit Polyclonal
Species Human, Mouse
Applications ICC/IF, IHC, IHC-P

8 Publications
MOB00B
 Leptin/OB [HRP] Leptin/OB [HRP]


Species Mouse, Rat
Applications ELISA

     5 Reviews

154 Publications