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Acylation Bioinformatics

Acylation is a type of post-translational modification (PTM) that involves the attachment of an acyl group to a protein's amino acid residues. Proteins may be acylated by covalently bonding various fatty acids. Acylation has an impact on protein function and intracellular localization.

Acylation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Acylation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1788 products for the study of Acylation that can be applied to Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry from our catalog of antibodies and ELISA kits.

Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)

Mouse Monoclonal
Species Human, Mouse, Porcine
Applications WB, Flow, ICC/IF

     5 Reviews

Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.

Rat Monoclonal
Species Mouse
Applications WB, Flow, IA

     2 Reviews

2 Publications
Western Blot: Ghrelin Receptor/GHSR Antibody [NBP2-16655] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GHS-R1 antibody [N1], N-term  diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunohistochemistry-Paraffin: GHSR Antibody [NBP2-16655] - Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

    Western  blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF  membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone  Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by  HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=    Recombinant  Human Growth Hormone protein was serially diluted 2-fold and captured by  Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, IHC

     3 Reviews

6 Publications
3 Publications
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.

Goat Polyclonal
Species Mouse
Applications WB, ELISA, ICC/IF

41 Publications
Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.1 µg/lane of Recombinant Human TNF-alpha  was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

Species Human

     24 Reviews

584 Publications
Western Blot: Acetylcholinesterase/ACHE Antibody (ZR3) [NBP2-22449] - Western blot analysis was performed on whole cell extracts (30 ug lysate) of Mouse Brain (lane 1), HeLa (lane 2), SK-N-SH (lane 3) and U-87 MG (lane 4).Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody (ZR3) [NBP2-22449] - Analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) shows staining in U251 glioma cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Acetylcholinesterase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

Western Blot: ATG5 Antibody [NB110-53818] - Analysis in mouse wildtype ES cell lysate (Lane 1) using NB110-53818. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.Western Blot: ATG5 Antibody [NB110-53818] - Total protein from Human HeLa and A431 and Mouse MEF cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, EM

     6 Reviews

175 Publications
Western Blot: ASPA Antibody [NBP1-31754] -  Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aspartoacylase antibody  diluted by 1:1000.Immunocytochemistry/Immunofluorescence: ASPA Antibody [NBP1-31754] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aspartoacylase protein stained by  partoacylase antibody [N1C3-2] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody  diluted at 1:1000. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

Recombinant Human ASIP/Agouti (Catalog # 9094-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED<sub>50</sub> for this effect is 0.8-4 μg/mL.

Species Human

Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250.  Detection:  Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.

Rabbit Polyclonal
Species Human, Mouse
Applications ICC/IF, IHC, IHC-P

5 Publications
Western Blot: Ropporin 1-like Antibody [NBP1-90081] - Analysis in control (vector only transfected HEK293T lysate) and ROPN1L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: Ropporin 1-like Antibody [NBP1-90081] - Staining of human cerebral cortex.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
Western Blot: ACF Antibody [NBP1-90271] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG spImmunohistochemistry-Paraffin: ACF Antibody [NBP1-90271] - Staining of human colon shows moderate nuclear/cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

Western Blot: NDUFAB1 Antibody [NBP1-31682] - 30 ug PC-12 whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:5000.Immunocytochemistry/Immunofluorescence: NDUFAB1 Antibody [NBP1-31682] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

Western Blot: GNPDA1 Antibody [NBP2-24557] - analysis of GNPDA1 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. 25 ug/ml.Immunohistochemistry-Paraffin: GNPDA1 Antibody [NBP2-24557] - Formalin-fixed paraffin-embedded human kidney tissue stained with GNPDA1 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Rabbit Polyclonal
Species Human, Equine, Primate
Applications WB, IHC-P