ZMIZ1/Zimp10 Antibody

Images

 
Western Blot: ZMIZ1/Zimp10 Antibody [NBP1-76553] - K562 cell lysate with ZIMP10 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry/ Immunofluorescence: ZMIZ1/Zimp10 Antibody [NBP1-76553] - Immunocytochemistry of ZIMP10 in K562 cells with ZIMP10 antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: ZMIZ1/Zimp10 Antibody [NBP1-76553] - Immunofluorescence of ZIMP10 in K562 cells with ZIMP10 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ZMIZ1/Zimp10 Antibody Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIMP10. The immunogen is located within amino acids 90 - 140 of ZIMP10.
Specificity
At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members.
Predicted Species
Mouse (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZMIZ1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
Control Peptide

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for ZMIZ1/Zimp10 Antibody

  • FLJ13541
  • hZIMP10
  • KIAA1224
  • KIAA1224Zimp10
  • MIZ
  • PIAS-like protein Zimp10
  • RAI17
  • RAI17TRAFIP10
  • retinoic acid induced 17
  • Retinoic acid-induced protein 17
  • RP11-519K18.1
  • TRAFIP10
  • Zimp10
  • ZIMP10FLJ00092 protein
  • zinc finger MIZ domain-containing protein 1
  • zinc finger, MIZ-type containing 1
  • zinc finger-containing, Miz1, PIAS-like protein on chromosome 10
  • ZMIZ1

Background

ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZMIZ1
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