ZAP70 [p Tyr493] Antibody

Images

 
Western Blot: ZAP70 Antibody [NBP1-51432] - Analyzes of p-ZAP70 (Y493) pAb in extracts from Jurkat cells.
Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-51432] - Analyzes of p-ZAP70 (Y493) pAb in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51432
    • Availability
      Product Discontinued

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ZAP70 [p Tyr493] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of Tyrosine 493.
Modification
p Tyr493
Specificity
p-ZAP-70 (Y493) pAb detects endogenous levels of ZAP-70 protein only when phosphorylated at Y493.
Clonality
Polyclonal
Host
Rabbit
Gene
ZAP70
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-ZAP-70 (Y493) pAb in extracts from Jurkat cells.
Immunohistochemistry (IHC) analyzes of p-ZAP-70 (Y493) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZAP70 [p Tyr493] Antibody

  • 70 kDa zeta-associated protein
  • EC 2.7.10
  • EC 2.7.10.2
  • mrtle
  • mur
  • SRK
  • SRKFLJ17679
  • STD
  • STDFLJ17670
  • Syk-related tyrosine kinase
  • tyrosine-protein kinase ZAP-70
  • TZK
  • ZAP70
  • ZAP-70
  • zeta-chain (TCR) associated protein kinase (70 kD)
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain associated protein kinase, 70kD

Background

The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, δ and epsilon chains and a zeta -containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ZAP70
Entrez