ZAP70 Antibody

Images

 
Western Blot: ZAP-70 Antibody [NB100-92241] - Western blot (WB) analysis of ZAP-70 (T286) antibody in extracts from 293 cells treated with PMA 125ng/ml 30

Product Details

Summary
Product Discontinued
View other related ZAP70 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92241
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

ZAP70 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 250-300 of Human ZAP70.
Clonality
Polyclonal
Host
Rabbit
Gene
ZAP70
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of ZAP-70 (T286) pAb in extracts from 293 cells treated with PMA 125ng/ml 30'.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZAP70 Antibody

  • 70 kDa zeta-associated protein
  • EC 2.7.10
  • EC 2.7.10.2
  • mrtle
  • mur
  • SRK
  • SRKFLJ17679
  • STD
  • STDFLJ17670
  • Syk-related tyrosine kinase
  • tyrosine-protein kinase ZAP-70
  • TZK
  • ZAP70
  • ZAP-70
  • zeta-chain (TCR) associated protein kinase (70 kD)
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain associated protein kinase, 70kD

Background

The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and episilon chains and a zeta-containing homodimer or heterodimer. The disulfide-linked Ti alpha-beta heterodimer is responsible for antigen recognition, but the short five amino acid cytoplasmic domains of Ti alpha and beta are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and zeta subunits suggest a role in signal transduction. Of these, the zeta chain, which is expressed as either a homodimer or heterodi-mer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A 70 kDa tyrosine phosphoprotein, ZAP-70, has been identified that associates with zeta and undergoes tyrosine phosphorylation following TCR stimulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB7500
Species: Hu
Applications: WB, ICC
NBP2-24867
Species: Hu, Mu
Applications: Flow, Func, In vitro, In vivo, CyTOF-ready, Flow-CS
NBP2-25250
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-32945
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-27104
Species: Hu, Mu, Pm
Applications: WB, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-46177
Species: Hu
Applications: IP (-), WB, IHC, IHC-P
NBP1-92440
Species: Hu
Applications: WB, IHC, IHC-P
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NB500-517
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
MAB37861
Species: Hu
Applications: WB, Simple Western, ICC
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB7474
Species: Hu
Applications: WB, Simple Western, ICC
NBP2-37574
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC
NB100-92241
Species: Hu, Mu
Applications: WB

Publications for ZAP70 Antibody (NB100-92241) (0)

There are no publications for ZAP70 Antibody (NB100-92241).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ZAP70 Antibody (NB100-92241) (0)

There are no reviews for ZAP70 Antibody (NB100-92241). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ZAP70 Antibody (NB100-92241) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ZAP70 Products

Bioinformatics Tool for ZAP70 Antibody (NB100-92241)

Discover related pathways, diseases and genes to ZAP70 Antibody (NB100-92241). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ZAP70 Antibody (NB100-92241)

Discover more about diseases related to ZAP70 Antibody (NB100-92241).
 

Pathways for ZAP70 Antibody (NB100-92241)

View related products by pathway.

PTMs for ZAP70 Antibody (NB100-92241)

Learn more about PTMs related to ZAP70 Antibody (NB100-92241).
 

Research Areas for ZAP70 Antibody (NB100-92241)

Find related products by research area.

Blogs on ZAP70

There are no specific blogs for ZAP70, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ZAP70 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ZAP70
Entrez