ZAP70 Antibody (1E7.2)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Clone
1E7.2
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

ZAP70 Antibody (1E7.2) Summary

Immunogen
A KLH-coupled peptide corresponding to amino acids 282-307 of human ZAP-70
Specificity
The mouse monoclonal antibody 1E7.2 recognizes ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ZAP70
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for ZAP70 Antibody (1E7.2)

  • 70 kDa zeta-associated protein
  • EC 2.7.10
  • EC 2.7.10.2
  • mrtle
  • mur
  • SRK
  • SRKFLJ17679
  • STD
  • STDFLJ17670
  • Syk-related tyrosine kinase
  • tyrosine-protein kinase ZAP-70
  • TZK
  • ZAP70
  • ZAP-70
  • zeta-chain (TCR) associated protein kinase (70 kD)
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain associated protein kinase, 70kD

Background

The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZAP70 Antibody (NBP1-50059) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-50059AF405
Alexa Fluor 488 Labeled NBP1-50059AF488
Alexa Fluor 647 Labeled NBP1-50059AF647
Alexa Fluor 700 Labeled NBP1-50059AF700
Allophycocyanin Labeled NBP1-50059APC
DyLight 350 Labeled NBP1-50059UV
DyLight 405 Labeled NBP1-50059V
DyLight 405LS Labeled NBP1-50059V3
DyLight 488 Labeled NBP1-50059G
DyLight 680 Labeled NBP1-50059FR
DyLight 755 Labeled NBP1-50059IR
PE Labeled NBP1-50059PE
PerCP Labeled NBP1-50059PCP

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol ZAP70
Entrez
Uniprot

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