YAP1 Antibody


Western Blot: YAP1 Antibody [NB100-92344] - YAP (Q295) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:Rat kidney tissue lyate
Immunohistochemistry-Paraffin: YAP1 Antibody [NB100-92344] - Paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: YAP1 Antibody [NB100-92344] - Western blot (WB) analysis of YAP1 (Q295) antibody in extracts from HeLa cells.
Immunohistochemistry-Paraffin: YAP1 Antibody [NB100-92344] - Human breast carcinoma tissue

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YAP1 Antibody Summary

Synthetic peptide, corresponding to amino acids 280-330 of Human YAP.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of YAP1 (Q295) pAb in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of YAP1 (Q295) pAb in paraffin-embedded human breast carcinoma tissue. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23998984)
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
YAP1 Lysate (NBP2-65458)
Read Publications using
NB100-92344 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for YAP1 Antibody

  • 65 kDa Yes-associated protein
  • YAP
  • YAP1
  • YAP2
  • YAP65
  • YAP65yes-associated protein 2
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • YKI
  • Yorkie Homolog


The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for YAP1 Antibody (NB100-92344)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, IHC-Fr.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies


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Blogs on YAP1.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

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Gene Symbol YAP1