YAP1 Antibody

Images

 
Western Blot: YAP1 Antibody [NB100-92344] - YAP (Q295) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:Rat kidney tissue lyate
Immunohistochemistry-Paraffin: YAP1 Antibody [NB100-92344] - Paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: YAP1 Antibody [NB100-92344] - Western blot (WB) analysis of YAP1 (Q295) antibody in extracts from HeLa cells.
Immunohistochemistry-Paraffin: YAP1 Antibody [NB100-92344] - Human breast carcinoma tissue

Product Details

Summary
Product Discontinued
View other related YAP1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92344
    • Availability
      Product Discontinued

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YAP1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 280-330 of Human YAP.
Clonality
Polyclonal
Host
Rabbit
Gene
YAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of YAP1 (Q295) pAb in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of YAP1 (Q295) pAb in paraffin-embedded human breast carcinoma tissue. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23998984)
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-92344 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for YAP1 Antibody

  • 65 kDa Yes-associated protein
  • YAP
  • YAP1
  • YAP2
  • YAP65
  • YAP65yes-associated protein 2
  • Yes-associated protein 1
  • Yes-associated protein 1, 65kDa
  • yes-associated protein beta
  • yes-associated protein delta
  • YKI
  • Yorkie Homolog

Background

The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for YAP1 Antibody (NB100-92344)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, IHC-Fr.


Filter By Application
ICC/IF
(2)
IHC-Fr
(1)
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Mouse
(1)
Rat
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for YAP1 Antibody (NB100-92344)

Discover related pathways, diseases and genes to YAP1 Antibody (NB100-92344). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for YAP1 Antibody (NB100-92344)

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Blogs on YAP1.

YAP1 - a transcription co-activator and the downstream target of Hippo pathway
YAP1 (Yes-associated protein 1) is a  transcriptional co-activator which acts as a major effector of Hippo signaling pathway that regulates organ size/ tissue homeostasis and cell proliferation, and is an established oncogene (1).  Hippo sig...  Read full blog post.

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Bioinformatics

Gene Symbol YAP1
Entrez