XRCC4 Antibody

Images

 
Western Blot: XRCC4 Antibody [NBP1-31339] - Analysis of NBP1-31339 on 293T (lane A), A431 (lane B), H1299 (lane C), HeLa (lane D), Hep G2 (lane E), MOLT4 (lane F), and Raji (lane G), with 10% SDS PAGE, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: XRCC4 Antibody [NBP1-31339] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-31339] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
Western Blot: XRCC4 Antibody [NBP1-31339] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 C: HepG2 D: Molt-4 E: Raji 10% SDS PAGE, antibody diluted at 1:2000.
Immunoprecipitation: XRCC4 Antibody [NBP1-31339] - XRCC4 antibody immunoprecipitates XRCC4 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.39 mg/ml

Order Details

XRCC4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human XRCC4. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XRCC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.39 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XRCC4 Antibody

  • complementing defective, repair in Chinese hamster
  • X-ray repair complementing defective repair in Chinese hamster cells 4
  • X-ray repair cross-complementing protein 4

Background

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. This gene contains 8 exons, and alternative transcription initiation and alternative splicing generates several transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for XRCC4 Antibody (NBP1-31339)

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Blogs on XRCC4.

Ku70: The DNA's Mr. Fix-it
Ku70, known by several synonyms including X-ray repair cross-complementing, 5'-deoxyribose-5-phosphate lyase Ku70 protein 6, 70 kDa subunit of Ku antigen, XRCC6, and G22P1, is a 70 kDa protein that was shown to be involved in multiple cellular pathway...  Read full blog post.

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Bioinformatics

Gene Symbol XRCC4