XRCC1 Antibody (2F8)

Images

 
Western Blot: XRCC1 Antibody (2F8) [NBP2-02280] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: XRCC1 Antibody (2F8) [NBP2-02280] Staining of COS7 cells transiently transfected by pCMV6-ENTRY XRCC1.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - staining of paraffin-embedded bladder tissue using anti-RC204952 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded colon tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded Kidney tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded lung tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded pancreas tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: XRCC1 Antibody (2F8) [NBP2-02280] - Staining of paraffin-embedded prostate tissue using anti-XRCC1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
2F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

XRCC1 Antibody (2F8) Summary

Immunogen
Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XRCC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for XRCC1 Antibody (2F8)

  • DNA repair protein XRCC1
  • RCC
  • X-ray repair complementing defective repair in Chinese hamster cells 1
  • X-ray repair cross-complementing protein 1
  • X-ray-repair, complementing defective, repair in Chinese hamster

Background

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Bioinformatics

Gene Symbol XRCC1
Entrez