XPF Antibody (OTI2A1)


Western Blot: XPF Antibody (2A1) [NBP2-01059] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more
Immunocytochemistry/ Immunofluorescence: XPF Antibody (2A1) [NBP2-01059] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF.
Flow Cytometry: XPF Antibody (2A1) [NBP2-01059] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XPF antibody, and then analyzed by ...read more
Western Blot: XPF Antibody (2A1) [NBP2-01059] Analysis of extracts (35ug) from 9 different cell lines by using anti-XPF monoclonal antibody.

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XPF Antibody (OTI2A1) Summary

Full length human recombinant protein of human ERCC4(NP_005227) produced in HEK293 cell.
Immunogen affinity purified
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  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human, Dog, Rat, Monkey, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for XPF Antibody (OTI2A1)

  • DNA excision repair protein ERCC-4
  • DNA repair protein complementing XP-F cells
  • EC 3.1
  • ERCC11
  • ERCC4
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 4
  • xeroderma pigmentosum, complementation group F
  • XPF
  • XPFcomplementing defective, in Chinese hamster


The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Blogs on XPF.

NER Antibodies in Cancer Research
We at Novus Biologicals have over 230 products in our antibody catalog devoted to nucleotide excision repair. NER is a multi-stage sequential process involving over 30 proteins, all of which have been widely studied. Being the primary method to repair...  Read full blog post.

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Gene Symbol ERCC4