XPB Antibody

Images

 
Western Blot: XPB Antibody [NBP1-31389] - XPB antibody detects XPB protein by Western blot analysis.A. 30 ug Neuro2A whole cell lysate/extractB. 30 ug GL261 whole cell lysate/extractC. 30 ug C8D30 whole cell ...read more
Immunocytochemistry/ Immunofluorescence: XPB Antibody [NBP1-31389] - Analysis of HeLa, using NBP1-31389 at 1:200 dilution.
Western Blot: XPB Antibody [NBP1-31389] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Mk, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.69 mg/ml

Order Details

XPB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human XPB. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (99%), Chicken (93%), Monkey (100%), Bovine (100%), Zebrafish (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ERCC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.69 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XPB Antibody

  • Basic transcription factor 2 89 kDa subunit
  • BTF2 p89
  • BTF2
  • DNA excision repair protein ERCC-3
  • DNA repair protein complementing XP-B cells
  • EC 3.6.1
  • EC 3.6.4.12
  • ERCC3
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 3 (xeroderma pigmentosum group B complementing)
  • GTF2H
  • RAD25
  • TFIIH basal transcription factor complex 89 kDa subunit
  • TFIIH basal transcription factor complex helicase XPB subunit
  • TFIIH p89
  • TFIIH
  • Xeroderma pigmentosum group B-complementing protein
  • xeroderma pigmentosum, complementation group B
  • XPB
  • XPBC
  • XPBTFIIH 89 kDa subunit

Background

ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ERCC3
Entrez