XLF Antibody (OTI1E2)


Western Blot: XLF Antibody (1E2) [NBP2-03402] Analysis of extracts (35ug) from 9 different cell lines by using anti-XLF monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: XLF Antibody (1E2) [NBP2-03402] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XLF.
Immunohistochemistry-Paraffin: XLF Antibody (1E2) [NBP2-03402] - Staining of paraffin-embedded Human liver tissue using anti-XLF mouse monoclonal antibody.
Flow Cytometry: XLF Antibody (1E2) [NBP2-03402] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-XLF antibody, and then analyzed by ...read more
Western Blot: XLF Antibody (1E2) [NBP2-03402] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XLF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more

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XLF Antibody (OTI1E2) Summary

Full length recombinant protein of human XLF (NP_079058) produced in HEK293T cells.
Immunogen affinity purified
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  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
XLF Lysate (NBP2-65419)

Reactivity Notes

Human, Dog, Rat, Monkey

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for XLF Antibody (OTI1E2)

  • Cernunnos
  • non-homologous end-joining factor 1
  • nonhomologous end-joining factor 1
  • Protein cernunnos
  • XLFFLJ12610
  • XRCC4-like factor


Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


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Bioinformatics Tool for XLF Antibody (NBP2-03402)

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Diseases for XLF Antibody (NBP2-03402)

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Research Areas for XLF Antibody (NBP2-03402)

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Blogs on XLF.

Ku70: The DNA's Mr. Fix-it
Ku70, known by several synonyms including X-ray repair cross-complementing, 5'-deoxyribose-5-phosphate lyase Ku70 protein 6, 70 kDa subunit of Ku antigen, XRCC6, and G22P1, is a 70 kDa protein that was shown to be involved in multiple cellular pathway...  Read full blog post.

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Gene Symbol NHEJ1