WWOX Antibody

Product Discontinued

WWOX Antibody Summary

• Immunogen: A synthetic peptide corresponding to residues 89-107 [RLAFTVDDNPTKPTTRQRY] of the human protein.
• Specificity: WW domain-containing oxidoreductase
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WWOX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:100-1000
  • Immunohistochemistry-Frozen 1:100-1000
  • Immunohistochemistry-Paraffin 1:100-1000
  • Immunoprecipitation 3ug / ml
  • Western Blot 1:500-5000
• Control:

  WWOX Overexpression Lysate

Reactivity Notes

Reacts with human, mouse, chimpanzee and orangutang.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WWOX Antibody

• D16S432E
• EC 1.1.1
• EC 1.1.1.-
• FOR
• FORHHCMA56
• FRA16D
• Fragile site FRA16D oxidoreductase
• SDR41C1
• short chain dehydrogenase/reductase family 41C, member 1
• WOX1
• WOX1PRO0128
• WW domain containing oxidoreductase
• WW domain-containing oxidoreductase
• WW domain-containing protein WWOX
• WWOX

Background

WW domain-containing oxidoreductase (WWOX) is a proapoptotic protein and a tumor suppressor gene. Because mutation or deletion in the coding region of WWOX is rarely found, it is speculated that the appearance of aberrant transcripts affects progression of many cancers. In addition, protein levels of WWOX, its Tyr33-phosphorylated form, and WOX2 are significantly down-regulated in the neurons of Alzheimer's hippocampi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WWOX Antibody (NB200-178)(5)

Publications using NB200-178

• Bendinelli P, Maroni P, Matteucci E, Desiderio MA. HGF and TGFB1 differently influenced Wwox regulatory function on Twist program for mesenchymal-epithelial transition in bone metastatic versus parental breast carcinoma cells Mol. Cancer. 2015-06-04 [PMID: 26041563] (IHC-P, Human)
  
  Details:
  WWOX antibody (Imgenex: IMG-4187) was used at 1:200 dilution on sections from human bone metastasis and primary breast carcinoma [serial sections of pair-matched human specimens from primary-invasive ductal breast carcinoma (positive for oestrogen and progesterone receptors and negative for HER2) and from bone metastasis] (Fig. 7), as well as from bone metastasis and the bone marrow of the 1833 cells xenograft model (Figure S3).
• Winardi W, Tsai CY, Chen WT et al. Reduced WWOX protein expression in human astrocytoma. Neuropathology 2013-12-01 [PMID: 23675860] (IHC-P, Human)
• Li YP, Wu CC, Chen WT et al. The expression and significance of WWOX and B-catenin in hepatocellular carcinoma. APMIS. 2013-02-01 [PMID: 23030478]
• Maeda N, Semba S, Nakayama S et al. Loss of WW domain-containing oxidoreductase expression in the progression and development of gastric carcinoma: clinical and histopathologic correlations. Virchows Arch. 2010-10-01 [PMID: 20737170] (IF, IHC-P, WB, Human)
  
  Details:
  WB: Fig 1B (normal gastric mucosa and gastrocarcinoma (GC) cell lines, Fig 2B (GC), Fig 3A (Adenovirus-WWOX infected HSC-59 GC cells). IF: Fig 1C (GC cells). IHC (P): Fig 4A (GC human tumors and normal gastric mucosa), Table 1 (WWOX expression correlation
• Nakayama S, Semba S, Maeda N et al. Hypermethylation-mediated reduction of WWOX expression in intraductal papillary mucinous neoplasms of the pancreas. Br J Cancer. 2009-05-05 [PMID: 19352382] (IHC-P, Human)
  
  Details:
  IHC (P): Human pancreatic tumor tissue (Figs 1 and 3; Tables I and II).

FAQs for WWOX Antibody (NB200-178). (Showing 1 - 1 of 1 FAQs).

1. I have a question on anti-WWOX antibody NB200-178. How was the antibody purified and what proteins are present in the product as it is offered. Is it purely IgG?
   • NB200-178 is immunogen affinity purified and is supplied in PBS with 0.02% sodium azide. It does not contain any carrier proteins so the rabbit antibody is the only protein expected to be present.

Bioinformatics

• Gene Symbol: WWOX
• Entrez:
  • 51741(Human)
  • 80707(Mouse)
• Uniprot:
  • Q9NZC7()