WT1 Antibody

Product Discontinued

WT1 Antibody Summary

• Immunogen: A synthetic peptide derived from the C-terminus of human Wilm's Tumor protein.
• Localization: Nucleus
• Specificity: This product is specific for Wilm's Tumor Protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Predicted cross-reactivity based on sequence identity: Mouse, Rat, Rabbit, Dog, Pig.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.6) and 1.0% BSA
• Preservative: 0.099% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for WT1 Antibody

• AWT1
• GUD
• NPHS4GUD
• WAGR
• Wilms tumor 1
• Wilms tumor protein
• WIT-2
• WT1
• WT33

Background

Wilm's Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilm's tumors and highly expressed in a wide variety of other malignancies (1). It is believed that WT in its two isoforms acts as both a transcription factor and a post-transcriptional regulator that binds RNA and can interact with splicing components (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WT1
• Entrez:
  • 7490(Human)
• Uniprot:
  • P19544(Human)