WDHD1 [p Ser1041] Antibody (20G10)

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Western Blot: WDHD1 [p Ser1041] Antibody (20G10) [NBP1-48696] - Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with ...read more
Immunohistochemistry-Paraffin: WDHD1 [p Ser1041] Antibody (20G10) [NBP1-48696] - Formalin-fixed paraffin-embedded human kidney tissue was stained with 20G10 at 15 ug/ml and developed with an alkaline phosphatase ...read more

Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-48696
    • Availability
      Product Discontinued

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WDHD1 [p Ser1041] Antibody (20G10) Summary

Immunogen
This antibody was generated by immunizing host with Recombinant (partial), C-terminal
Modification
p Ser1041
Localization
Found in the nucleus and nucleolus. Expressed in brain, epidermis, liver, and stomach. During mitosis transiently expressed in nucleoplasm.
Specificity
This antibody has been tested to recognize phosphorylated and nonphosphorylated forms. Post translational modifications exist on site S1041.The modifications consist of Phosphorylation.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WDHD1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for WDHD1 [p Ser1041] Antibody (20G10)

  • Acidic nucleoplasmic DNA-binding protein 1
  • AND1
  • AND-1
  • CHTF4
  • CTF4
  • CTF4, chromosome transmission fidelity factor 4 homolog
  • WD repeat and HMG-box DNA binding protein 1
  • WD repeat and HMG-box DNA-binding protein 1

Background

The 20G10 monoclonal antibody recognizes both human and mouse AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be in chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. The 20G10 antibody has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol WDHD1