WASF1/WAVE1 [p Tyr125] Antibody

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Immunohistochemistry: WASF1 [p Tyr125] Antibody [NB100-92708] - Immunohistochemistry (IHC) analysis of WASF1 [p Tyr125] antibody in paraffin-embedded human colon muscle tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92708
    • Availability
      Product Discontinued

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WASF1/WAVE1 [p Tyr125] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human WAVE1 around the phosphorylation site of Tyrosine 125.
Modification
p Tyr125
Clonality
Polyclonal
Host
Rabbit
Gene
WASF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of WASF1 [p Tyr125] pAb in extracts from NIH-3T3 cells.
Immunohistochemistry (IHC) analyzes of WASF1 [p Tyr125] pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
WASF1/WAVE1 Lysate (NBL1-17775)

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WASF1/WAVE1 [p Tyr125] Antibody

  • KIAA0269WAVEhomology of dictyostelium scar 1
  • Protein WAVE-1
  • SCAR1
  • SCAR1WASP family protein member 1
  • Verprolin homology domain-containing protein 1
  • WAS protein family, member 1
  • WASF1
  • WASP1
  • WAVE1
  • WAVE1FLJ31482
  • wisk

Background

WASP (for Wiskott-Aldrich syndrome protein), N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WASF1
Entrez
Uniprot