VPS34 Antibody

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Western Blot: VPS34 Antibody [NBP1-32545] - Non-transfected ( -) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [C1C3].
Western Blot: VPS34 Antibody [NBP1-32545] - (30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.02 mg/ml
     

Genetic Strategies

   

Order Details

VPS34 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human VPS34. The exact sequence is proprietary.
Predicted Species
Mouse (99%), Rat (99%), Porcine (100%), Xenopus (95%), Zebrafish (91%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIK3C3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.02 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS34 Antibody

  • EC 2.7.1
  • EC 2.7.1.137
  • hVps34
  • MGC61518
  • phosphatidylinositol 3-kinase catalytic subunit type 3
  • Phosphatidylinositol 3-kinase p100 subunit
  • Phosphoinositide-3-kinase class 3
  • phosphoinositide-3-kinase, class 3
  • PI 3-Kinase C3
  • PI3K type 3
  • PI3-kinase type 3
  • PIK3C3
  • PtdIns-3-kinase type 3
  • VPS34

Background

VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for VPS34 Antibody (NBP1-32545). (Showing 1 - 1 of 1 FAQ).

  1. Hi, could you tell us, what the lower band in the WB picture of the PIK3c3 antibody (at ~70kDA) is?
    • The lower band at ~75kDa that is observed in the WB has not been identified as of yet. I checked with our lab team and they have confirmed that they have observed this on numerous occasions however, the origin has not been determined. The PIK3C3 band that is the one at ~120kDa.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for VPS34 Antibody (NBP1-32545)

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Blogs on VPS34.

UVRAG - A regulator of membrane trafficking in autophagy and endocytosis
UV resistance-associated gene (UVRAG) is a tumor suppressor that is commonly mutated in colon and breast cancer. While UVRAG was discovered for its ability to complement UV sensitivity in xeroderma pigmentosum cells, its main functions are in auto...  Read full blog post.

VPS34 - autophagy initiator and regulator of endosomal trafficking
VPS34, vacuolar protein sorting 34, is the only identified Class III phosphoinositide-3 kinase (PI3K) in mammals and is ubiquitously expressed in all eukaryotic cells. VPS34 is a 100 kDa protein responsible for phosphorylating phosphatidylinositol...  Read full blog post.

ULK1 - mammalian homologue of the yeast ATG1 kinase
Autophagy is an important cellular process involved in degradation and recycling of cellular macromolecules in response to stress or starvation. Autophagy is carried out in four main phases: phagophore nucleation, autophagosome elongation, docking...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

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Bioinformatics

Gene Symbol PIK3C3
Entrez