VPS34 Antibody (EPR5301)

Product Discontinued

VPS34 Antibody (EPR5301) Summary

• Immunogen: A synthetic peptide corresponding to residues in human PI3-Kinase Class 3 (VPS34) was used as an immunogen.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PIK3C3
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-100
  • Western Blot 1:1000-10000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Species cross-reactivity is based on Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for VPS34 Antibody (EPR5301)

• EC 2.7.1
• EC 2.7.1.137
• hVps34
• MGC61518
• phosphatidylinositol 3-kinase catalytic subunit type 3
• Phosphatidylinositol 3-kinase p100 subunit
• Phosphoinositide-3-kinase class 3
• phosphoinositide-3-kinase, class 3
• PI 3-Kinase C3
• PI3K type 3
• PI3-kinase type 3
• PIK3C3
• PtdIns-3-kinase type 3
• VPS34

Background

PI3-Kinase Class 3 (VPS34) is the catalytic subunit of the PI3K complex. It is involved in the transport of lysosomal enzyme precursors to lysosomes and the induction of autophagy during nutrient deprivation (1, 2). Thus, PI3-Kinase Class 3 (VPS34) regulates intracellular vesicular transport at multiple steps through the production of phosphatidylinositol-3-phosphate (PI(3)P). Certain downstream effectors are involved in each of the processes that PI3-Kinase Class 3 (VPS34) regulates. It exerts its function through the recruitment of proteins that contain PI(3)P-binding motifs such as FYVE or PX domains (3). PI3-Kinase Class 3 (VPS34) is also required for the activation of the mTOR (mammalian target of rapamycin)/S6K1 (S6 kinase 1) pathway, which regulates protein synthesis in response to nutrient availability (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIK3C3
• Entrez:
  • 5289(Human)
  • 225326(Mouse)
  • 65052(Rat)
• Uniprot:
  • Q8NEB9(Human)