CCIEP : 20uL antiserum vs 10uL plasma, wells 6mm apart, rim to rim. 100v for 30 minutes Double Diffusion : 10uL antiserum vs 5uL VWF 1mg/mL Optimal dilutions should be determined by the individual laboratory. The use of 3% PEG 6000 with 1.2% agarose in a suitablebuffer (such as TBE or Tris-barbital pH 8.2) is recommended. Results can be enhanced by washing completed agarose gels in saline, drying and finallystaining with a protein stain such as coomassie blue. Suitability for use in enzyme-linked immunosorbent assays and Western blot has notbeen assessed but use in such assays should not necessarily be excluded. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 22628362)
Read Publication using NB120-8820 in the following applications:
Cross-reacts with Human.Not yet tested in other species.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Glycine ed saline (pH 7.4), 0.01% Benzamidine, 0.1% EACA and 1.0mM EDTA
0.099% Sodium Azide
Alternate Names for Von Willebrand Factor Antibody
coagulation factor VIII VWF
von Willebrand factor
Von Willebrand Factor (VWF) was previously known as Factor VIII related antigenis. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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