Von Willebrand Factor Antibody



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Von Willebrand Factor Antibody Summary

Human Von Willebrand Factor Antigen prepared from citrated human plasma. Greater than 95% purity by SDS-PAGE.
Secreted. Located in storage granules.
Endothelial Cell Marker
Shown to be specific by gel diffusion techniques.
IgG purified
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  • Immunocytochemistry/Immunofluorescence
  • Radioimmunodiffusion
  • Immunofluorescence
Application Notes
CCIEP : 20uL antiserum vs 10uL plasma, wells 6mm apart, rim to rim. 100v for 30 minutes Double Diffusion : 10uL antiserum vs 5uL VWF 1mg/mL Optimal dilutions should be determined by the individual laboratory. The use of 3% PEG 6000 with 1.2% agarose in a suitablebuffer (such as TBE or Tris-barbital pH 8.2) is recommended. Results can be enhanced by washing completed agarose gels in saline, drying and finallystaining with a protein stain such as coomassie blue. Suitability for use in enzyme-linked immunosorbent assays and Western blot has notbeen assessed but use in such assays should not necessarily be excluded. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 22628362)
Read Publication using
NB120-8820 in the following applications:

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Glycine ed saline (pH 7.4), 0.01% Benzamidine, 0.1% EACA and 1.0mM EDTA
0.099% Sodium Azide
IgG purified

Alternate Names for Von Willebrand Factor Antibody

  • coagulation factor VIII VWF
  • F8
  • F8VWF
  • von Willebrand factor
  • VWD
  • vWF


Von Willebrand Factor (VWF) was previously known as Factor VIII related antigenis. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Von Willebrand Factor Antibody (NB120-8820)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.

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Publication using NB120-8820 Applications Species
Shi Z, Neoh KG, Kang ET et al. Enhanced endothelial differentiation of adipose-derived stem cells by substrate nanotopography J Tissue Eng Regen Med (ICC/IF, Human)

This publication used the FITC conjugated form of this antibody (Cat# NB120-8822).
ICC/IF Human

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ICC/IF Video Protocol

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Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

Von Willebrand Factor: An important mediator and carrier of hemostasis
Human Von Willebrand Factor (factor VIII R: Ag) is a 270 kDa multimeric plasma gylcoprotein. Important in the maintenance of hemostasis, it mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation facto...  Read full blog post.

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Gene Symbol VWF