Von Willebrand Factor Antibody

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Product Details

Summary
Product Discontinued
View other related Von Willebrand Factor Primary Antibodies

Order Details


    • Catalog Number
      NB120-8820
    • Availability
      Product Discontinued

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Von Willebrand Factor Antibody Summary

Immunogen
Human Von Willebrand Factor Antigen prepared from citrated human plasma. Greater than 95% purity by SDS-PAGE.
Localization
Secreted. Located in storage granules.
Marker
Endothelial Cell Marker
Specificity
Shown to be specific by gel diffusion techniques.
Clonality
Polyclonal
Host
Sheep
Gene
VWF
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Radioimmunodiffusion
Application Notes
CCIEP : 20uL antiserum vs 10uL plasma, wells 6mm apart, rim to rim. 100v for 30 minutes Double Diffusion : 10uL antiserum vs 5uL VWF 1mg/mL Optimal dilutions should be determined by the individual laboratory. The use of 3% PEG 6000 with 1.2% agarose in a suitablebuffer (such as TBE or Tris-barbital pH 8.2) is recommended. Results can be enhanced by washing completed agarose gels in saline, drying and finallystaining with a protein stain such as coomassie blue. Suitability for use in enzyme-linked immunosorbent assays and Western blot has notbeen assessed but use in such assays should not necessarily be excluded. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 22628362)
Publications
Read Publication using
NB120-8820 in the following applications:

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Glycine ed saline (pH 7.4), 0.01% Benzamidine, 0.1% EACA and 1.0mM EDTA
Preservative
0.099% Sodium Azide
Purity
IgG purified

Alternate Names for Von Willebrand Factor Antibody

  • coagulation factor VIII VWF
  • F8
  • F8VWF
  • von Willebrand factor
  • VWD
  • vWF

Background

Von Willebrand Factor (VWF) was previously known as Factor VIII related antigenis. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Von Willebrand Factor Antibody (NB120-8820)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Human
(1)
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Showing Publication 1 - 1 of 1.
Publication using NB120-8820 Applications Species
Shi Z, Neoh KG, Kang ET et al. Enhanced endothelial differentiation of adipose-derived stem cells by substrate nanotopography J Tissue Eng Regen Med (ICC/IF, Human)

Details:
This publication used the FITC conjugated form of this antibody (Cat# NB120-8822).
ICC/IF Human

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Von Willebrand Factor Antibody (NB120-8820) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Von Willebrand Factor Antibody (NB120-8820)

Discover related pathways, diseases and genes to Von Willebrand Factor Antibody (NB120-8820). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Von Willebrand Factor Antibody (NB120-8820)

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Pathways for Von Willebrand Factor Antibody (NB120-8820)

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PTMs for Von Willebrand Factor Antibody (NB120-8820)

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Research Areas for Von Willebrand Factor Antibody (NB120-8820)

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Blogs on Von Willebrand Factor.

Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

Von Willebrand Factor: An important mediator and carrier of hemostasis
Human Von Willebrand Factor (factor VIII R: Ag) is a 270 kDa multimeric plasma gylcoprotein. Important in the maintenance of hemostasis, it mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation facto...  Read full blog post.

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Bioinformatics

Gene Symbol VWF
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