VIPR1/VPAC1 Antibody

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Western Blot: VIP Receptor 1 Antibody [NBP1-01051] - VIP Receptor 1 Antibody Western blot (WB) analysis of VPAC1 (V371) antibody in extracts from HT-29 cells

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-01051
    • Availability
      Product Discontinued

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VIPR1/VPAC1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 350-400 of Human VPAC1.
Clonality
Polyclonal
Host
Rabbit
Gene
VIPR1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of VPAC1 (V371) pAb in extracts from HT-29 cells.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VIPR1/VPAC1 Antibody

  • FLJ41949
  • HVR1
  • II
  • PACAP type II receptor
  • PACAP-R2
  • RDC1
  • type II
  • V1RG
  • vasoactive intestinal peptide receptor 1
  • vasoactive intestinal polypeptide receptor 1
  • VIP R1
  • VIP receptor, type I
  • VIPR
  • VIPR1
  • VPAC1
  • VPAC1R

Background

The vasoactive intestinal peptide (VIP) and the pituitary adenylate cylaseactivating polypeptide (PACAP) belong to a superfamily of peptide hormones that include glucagon, secretin and growth hormone releasing hormone. The effects of VIP and PACAP are mediated by three G-protein coupled receptors, VPAC1, VPAC2 and the PACAP receptor (also designated PAC1-R). The VPAC receptors have equal affinities for VIP and PACAP, which stimulate the activation of adenylyl cyclase. Both VPAC1, a 47 kDa protein, and VPAC2, a 65 kDa protein, are abundantly expressed in brain and T cells, where they modulate neuronal differentiation and T cell activation, respectively. The PACAP receptor is a seven transmembrane protein that produces at least eight isoforms by alternative splicing. Each isoform is associated with a specific signaling pathway and a specific expression pattern. The PACAP receptor, which is thought to play an integral role in brain development, preferentially binds PACAP in order to stimulate a cAMP-protein kinase A signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics Tool for VIPR1/VPAC1 Antibody (NBP1-01051)

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Bioinformatics

Gene Symbol VIPR1
Entrez
Uniprot
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