Vinculin Antibody (FB11)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42324
    • Availability
      Product Discontinued

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Vinculin Antibody (FB11) Summary

Immunogen
The antibody reacts with the Vinculin antigen.
Marker
Focal Adhesion Marker
Specificity
In immunoblotting the antibody identifies a 130 kD polypeptide. Monoclonal antibody to vinculin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Vinculin purified from human smooth muscle was used as an immunogen.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VCL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Immunohistochemical stainings on frozen sections, western blotting and other immunoassays for cellular and tumor biology.Working dilution in western blotting is at least 1:1000.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS solution containing 1.0% w/v BSA
Preservative
0.1% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Vinculin Antibody (FB11)

  • CMD1W
  • CMH15
  • metavinculin
  • MVCL
  • VCL
  • Vinculin

Background

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell extracellular matrix adherens type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha actinin and possibly with itself. Since alpha actinin binds to and cross links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Vinculin Antibody (NBP1-42324). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VCL
Entrez