vH RAS Overexpression Lysate (Native)

Images

 
Western Blot: vH RAS Overexpression Lysate (Adult Normal) [NBL1-11703] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for vH RAS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

vH RAS Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC216409 and based on accession number NM_005343. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1, mRNA.
Gene
HRAS

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for vH RAS

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for vH RAS Overexpression Lysate (Native)

  • C-BAS/HAS
  • C-HA-RAS1
  • c-has/bas p21 protein
  • C-H-RAS
  • c-ras-Ki-2 activated oncogene
  • CTLO
  • GTP- and GDP-binding peptide B
  • GTPase HRas
  • HAMSV
  • ha-Ras
  • Ha-Ras1 proto-oncoprotein
  • HRAS1
  • H-Ras-1
  • H-RASIDX
  • K-RAS
  • N-RAS
  • p19 H-RasIDX protein
  • p21ras
  • Ras family small GTP binding protein H-Ras
  • RASH1
  • transformation gene: oncogene HAMSV
  • Transforming protein p21
  • v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Background

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
H00003845-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP1-87425
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
MAB4540
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-33415
Species: Hu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBL1-11703
Species: Hu
Applications: WB

Publications for vH RAS Lysate (NBL1-11703) (0)

There are no publications for vH RAS Lysate (NBL1-11703).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for vH RAS Lysate (NBL1-11703) (0)

There are no reviews for vH RAS Lysate (NBL1-11703). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for vH RAS Lysate (NBL1-11703) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional vH RAS Products

Bioinformatics Tool for vH RAS Lysate (NBL1-11703)

Discover related pathways, diseases and genes to vH RAS Lysate (NBL1-11703). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for vH RAS Lysate (NBL1-11703)

Discover more about diseases related to vH RAS Lysate (NBL1-11703).
 

Pathways for vH RAS Lysate (NBL1-11703)

View related products by pathway.

PTMs for vH RAS Lysate (NBL1-11703)

Learn more about PTMs related to vH RAS Lysate (NBL1-11703).
 

Research Areas for vH RAS Lysate (NBL1-11703)

Find related products by research area.

Blogs on vH RAS

There are no specific blogs for vH RAS, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our vH RAS Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol HRAS