vH RAS Antibody (H-Ras-03)

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Western Blot: vH RAS Antibody (H-Ras-03) [NB110-68799] - Western Blotting analysis (reducing conditions) of vH RAS Antibody (H-Ras-03) in whole cell lysate using anti-human vH RAS Antibody (H-Ras-03). Lane 1: HeLa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
H-Ras-03
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

vH RAS Antibody (H-Ras-03) Summary

Immunogen
Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.
Specificity
The antibody H-RAS-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HRAS
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/ml
Application Notes
Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min. Application note: Reducing conditions. Purity is > 95% (by SDS-PAGE).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for vH RAS Antibody (H-Ras-03)

  • C-BAS/HAS
  • C-HA-RAS1
  • c-has/bas p21 protein
  • C-H-RAS
  • c-ras-Ki-2 activated oncogene
  • CTLO
  • GTP- and GDP-binding peptide B
  • GTPase HRas
  • HAMSV
  • ha-Ras
  • Ha-Ras1 proto-oncoprotein
  • HRAS1
  • H-Ras-1
  • H-RASIDX
  • K-RAS
  • N-RAS
  • p19 H-RasIDX protein
  • p21ras
  • Ras family small GTP binding protein H-Ras
  • RASH1
  • transformation gene: oncogene HAMSV
  • Transforming protein p21
  • v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Background

H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases vH RAS Antibody (H-Ras-03) affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of vH RAS Antibody (H-Ras-03) signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HRAS
Entrez
Uniprot