![SDS-Page: Recombinant Vespula vulgaris 5.0101 Protein [NBP3-07020] - Recombinant allergen rVes v 5 purity verification. 5 ug of rVes v 5 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.](http://images.novusbio.com/fullsize2/Recombinant-Vespula-vulgaris-5.0101-Protein-SDS-Page-NBP3-07020-img0001.jpg "SDS-Page: Recombinant Vespula vulgaris 5.0101 Protein [NBP3-07020] - Recombinant allergen rVes v 5 purity verification. 5 ug of rVes v 5 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.")
| Applications | ELISA, PAGE |
| Concentration | LYOPH |
| Description | Recombinant protein rVes v 5 is expressed in S2 cells (Drosophila). DNA sequence encoding 218 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 24.9 kDa. |
| Preparation Method |
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification. |
| Protein/Peptide Type | Recombinant Protein |
| Purity | >95%, by HPLC |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from a 0.22 um filtered solution in Tris buffer (100 mM Tris, 150 mM NaCl, pH 8), preservative- and carrier-free. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >95%, by HPLC |
| Reconstitution Instructions | Reconstitute with sterile deionized water with or without azide. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
