Recombinant Vespula vulgaris 1.0101 Strep (N-Term) Protein

SDS-Page: Recombinant Vespula vulgaris 1.0101 Protein [NBP3-07019] - Recombinant allergen rVes v 1 purity verification. 5 ug of rVes v 1 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

• Applications: WB, ELISA, Flow
• Concentration: LYOPH

Recombinant Vespula vulgaris 1.0101 Strep (N-Term) Protein Summary

• Description: Recombinant protein rVes v 1 is expressed in S2 cells (Drosophila). DNA sequence encoding 314 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 34.9 kDa.
• Preparation Method: The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by HPLC.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by HPLC.
• Reconstitution Instructions: Reconstitute with sterile deionized water with or without azide.

Alternate Names for Recombinant Vespula vulgaris 1.0101 Strep (N-Term) Protein

• Allergen Ves v I
• EC:3.1.1.32
• EC:3.1.1.4
• Phospholipase A1
• Ves v 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.