Human Versican ELISA Kit (Colorimetric)

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ELISA: Human Versican ELISA Kit (Colorimetric) [NBP2-31041]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Standard Curve Range
0.312-20ng/ml
Sensitivity
<32pg/ml

Order Details

Human Versican ELISA Kit (Colorimetric) Summary

Description
The Human Versican (VS) ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of VS in human plasma, serum, tissue homogenates and other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to VS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +- 10nm. The concentration of VS in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Specificity
This kit will detect isoforms V0, V1 and Vint.
Standard Curve Range
0.312-20ng/ml
Sensitivity
<32pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
<12%
Intra-Assay
<10%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
VCAN

Applications/Dilutions

Application Notes
Fragment encompassed by Thr1527-Pro1915

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Alternate Names for Human Versican ELISA Kit (Colorimetric)

  • Chondroitin sulfate proteoglycan 2WGN
  • Chondroitin sulfate proteoglycan core protein 2
  • CSPG2
  • CSPG2DKFZp686K06110
  • ERVR
  • GHAP
  • Glial hyaluronate-binding protein
  • Large fibroblast proteoglycan
  • PG-M
  • PG-MWGN1
  • VCAN
  • versican core protein
  • versican proteoglycan
  • Versican

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Versican ELISA Kit (NBP2-31041). (Showing 1 - 1 of 1 FAQ).

  1. Does this kit only detect Vo and V2?
    • This ELISA kit will detect Versican forms V0, V1 and Vint. The kit was generated using as immunogen, the fragment encompassed by Thr1527-Pro1915, UniProt accession number P13611.

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Bioinformatics

Gene Symbol VCAN
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