VEGFR1/Flt-1 Antibody (EIC)

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ELISA: VEGF R1/Flt-1 Antibody (EIC) [NB200-207] - Cross-reactivity of monoclonal and polyclonal anti-human Flt-1 antibodies with mouse Flt-1. Standard ELISA assay with coating of 2.5 ug/ml mouse sFlt-1-Fc.

Product Details

Summary
Product Discontinued
View other related VEGFR1/Flt-1 Primary Antibodies

Order Details


    • Catalog Number
      NB200-207
    • Availability
      Product Discontinued

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VEGFR1/Flt-1 Antibody (EIC) Summary

Immunogen
For immunization recombinant human soluble extracellular Flt-1 (D1-D5) native protein was used. The epitope is mapping at the amino terminus.
Localization
Type I membrane protein (Flt1) and soluble (SFlt1).
Marker
Endothelial Cell Marker
Specificity
The monoclonal will detect native and denaturated human VEGFR-1/Flt-1 1 in ELISA experiments, immunocytochemistry and cell sorting.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1.5-8 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 5-30 ug/ml
  • CyTOF-ready
Application Notes
analysis and cell sorting: The antibody has been used for the detection of Flt-1 positive cells (e.g. PAE/Flt-1 cells).
Positive Control
VEGFR1/Flt-1 Lysate (NBL1-06699)

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for VEGFR1/Flt-1 Antibody (EIC)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity. Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for VEGFR1/Flt-1 Antibody (NB200-207) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional VEGFR1/Flt-1 Products

Bioinformatics Tool for VEGFR1/Flt-1 Antibody (NB200-207)

Discover related pathways, diseases and genes to VEGFR1/Flt-1 Antibody (NB200-207). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for VEGFR1/Flt-1 Antibody (NB200-207)

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Pathways for VEGFR1/Flt-1 Antibody (NB200-207)

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PTMs for VEGFR1/Flt-1 Antibody (NB200-207)

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Bioinformatics

Gene Symbol FLT1
Entrez
Uniprot