VEGFR1/Flt-1 Antibody (7A6)

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Western Blot: VEGF Receptor 1 Antibody (7A6) [NBP2-00376] - Immunoblot analysis of non-boiled NP-40 lysates from Human Umbilical Vein Endothelial Cells (HUVEC) run on SDS-PAGE under non-reducing conditions. The membrane ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00376
    • Availability
      Product Discontinued

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VEGFR1/Flt-1 Antibody (7A6) Summary

Immunogen
The immunogen for this antibody is VEGF Receptor 1.
Marker
Endothelial Cell Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FLT1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This 7A6 antibody has been tested by immunoblot analysis of HUVEC. It can be used at 1-5 ug/ml. This antibody only recognizes non-reduced epitopes. It is highly recommended to include protease inhibitors in the lysing buffer and to not boil lysates prior to separation by nonreducing SDS-PAGE.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for VEGFR1/Flt-1 Antibody (7A6)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

This 7A6 monoclonal antibody recognizes human VEGFR1 (vascular endothelial growth factor receptor 1, fms-like tyrosine kinase 1, FLT1). It is directed against the extracellular domain and can recognize both membrane-bound and soluble forms of VEGFR1, with approximate molecular weights of 180 kDa and 90 kDa, respectively. Mice lacking expression of VEGFR1 have disorganized vasculature and die in utero, however angiogenesis does not require the tyrosine kinase activity of VEGFR1 as expression of a membrane-bound form lacking the tyrosine kinase domain allows normal development. Although fertile and viable, mice lacking the tyrosine kinase domain of VEGFR1 do have a defect in the migration of macrophages. The soluble form of VEGFR1 can be detected in the serum of normal patients, and elevated levels of soluble VEGFR1 in pregnant women is associated with preeclampsia. Several tumor cell lines have been reported to express membrane-bound VEGFR1, including malignancies of hematopoietic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Additional VEGFR1/Flt-1 Products

Bioinformatics Tool for VEGFR1/Flt-1 Antibody (NBP2-00376)

Discover related pathways, diseases and genes to VEGFR1/Flt-1 Antibody (NBP2-00376). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for VEGFR1/Flt-1 Antibody (NBP2-00376)

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Pathways for VEGFR1/Flt-1 Antibody (NBP2-00376)

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PTMs for VEGFR1/Flt-1 Antibody (NBP2-00376)

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Bioinformatics

Gene Symbol FLT1
Entrez