Human VEGF R3/Flt-4 ELISA Kit (Colorimetric)

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ELISA: VEGF Receptor 3 ELISA Kit 96Tests [NBP1-84822]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (citrate)
Standard Curve Range
0.6 - 40.0 ng/mL
Sensitivity
0.03 ng/mL

Order Details

Human VEGF R3/Flt-4 ELISA Kit (Colorimetric) Summary

Specificity
The assay detects both natural and recombinant human VEGF-R3/ FLT-4. Cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-R3/FLT-4 positive sample. There was no cross reactivity detected, notably not with VEGF-R1 and VEGF-R2/KDR. Interference was detected for VEGF-C at concentrations > 7 ng/ml, and not for VEGF-D.
Standard Curve Range
0.6 - 40.0 ng/mL
Sensitivity
0.03 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.0%
Intra-Assay
5.7%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
FLT4

Applications/Dilutions

Application Notes
The human VEGF-R3/FLT-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-R3/FLT-4

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human VEGF-R3/FLT-4
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Human VEGF-R3/FLT-4 Standard lyophilized, 80 ng/ml upon reconstitution
  5. Biotin-Conjugate anti-human VEGF-R3/FLT-4 monoclonal antibody
  6. Sample Diluent
  7. Stop Solution (1M Phosphoric acid)
  8. Substrate Solution (tetramethyl-benzidine)
  9. Streptavidin-HRP
  10. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human VEGF R3/Flt-4 ELISA Kit (Colorimetric)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol FLT4
Entrez