VEGF R3/Flt-4 Antibody [Unconjugated]

Images

 
bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGF R3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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VEGF R3/Flt-4 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF R3/Flt-4
Tyr25-Asp770
Accession # P35917
Specificity
Detects mouse VEGF R3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF R3, 10% cross-reactivity with rhVEGF R2, and 5% cross-reactivity with rhVEGF R1.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
Reviewed Applications
Read 3 Reviews rated 4
using
AF743 in the following applications:

Publications
Read Publications using
AF743 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF R3/Flt-4 Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGF R3 (Flt-4), together with VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

In adults, VEGF R3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGF R3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGF R3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

  1. Finnerty, H. et al. (1993) Oncogene 8:2293.
  2. Joukov, V. et al. (1996) EMBO J. 15:290.
  3. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGF R3/Flt-4 Antibody (AF743)(49)

We have publications tested in 2 confirmed species: Mouse, Equine.

We have publications tested in 14 applications: ELISA Development, ICC, IHC, IHC Frozen, IHC Fresh, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC paraffin embedded, IHC-Frozen, IHC-Paraffin-Embedded, IP, WB.


Filter By Application
ELISA Development
(1)
ICC
(3)
IHC
(22)
IHC Frozen
(1)
IHC Fresh
(4)
IHC Frozen
(5)
IHC Not Specified
(2)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(3)
IHC paraffin embedded
(1)
IHC-Frozen
(1)
IHC-Paraffin-Embedded
(1)
IP
(1)
WB
(2)
All Applications
Filter By Species
Mouse
(47)
Equine
(1)
All Species
Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using AF743 Applications Species
CA Thomson, SA van de Pav, M Stakenborg, E Labeeuw, G Matteoli, AM Mowat, RJB Nibbs Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators J. Immunol., 2018;0(0):. 2018 [PMID: 29760193] (IHC,, Mouse) IHC Mouse
M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program Nat Commun, 2018;9(1):1511. 2018 [PMID: 29666442] (IHC,, Mouse) IHC Mouse
Y Zhang, MH Ulvmar, L Stanczuk, I Martinez-C, M Frye, K Alitalo, T Mäkinen Heterogeneity in VEGFR3 levels drives lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms Nat Commun, 2018;9(1):1296. 2018 [PMID: 29615616] (ICC,, Mouse) ICC Mouse
J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28920924] (IHC,, Mouse) IHC Mouse
SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1 Sci Rep, 2017;7(1):4916. 2017 [PMID: 28687807] (IHC,, Mouse) IHC Mouse
D Olmeda, D Cerezo-Wal, E Riveiro-Fa, PC Pennacchi, M Contreras-, N Ibarz, M Cifdaloz, X Catena, TG Calvo, E Cañón, D Alonso-Cur, J Suarez, L Osterloh, O Graña, F Mulero, D Megías, M Cañamero, JL Martínez-T, C Mondal, J Di Martino, D Lora, I Martinez-C, JJ Bravo-Cord, J Muñoz, S Puig, P Ortiz-Rome, JL Rodriguez-, S Ortega, MS Soengas Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine Nature, 2017;546(7660):676-680. 2017 [PMID: 28658220] (IHC paraffin embedded,, Mouse) IHC paraffin embedded Mouse
F Tatin, E Renaud-Gab, AC Godet, F Hantelys, F Pujol, F Morfoisse, D Calise, F Viars, P Valet, B Masri, AC Prats, B Garmy-Susi Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction JCI Insight, 2017;2(12):. 2017 [PMID: 28614788] (IHC,, Mouse) IHC Mouse
P Yu, K Wilhelm, A Dubrac, JK Tung, TC Alves, JS Fang, Y Xie, J Zhu, Z Chen, F De Smet, J Zhang, SW Jin, L Sun, H Sun, RG Kibbey, KK Hirschi, N Hay, P Carmeliet, TW Chittenden, A Eichmann, M Potente, M Simons FGF-dependent metabolic control of vascular development Nature, 2017;0(0):. 2017 [PMID: 28467822] (IHC,, Mouse) IHC Mouse
JY Jang, SY Choi, I Park, DY Park, K Choe, P Kim, YK Kim, BJ Lee, M Hirashima, Y Kubota, JW Park, SY Cheng, A Nagy, YJ Park, K Alitalo, M Shong, GY Koh VEGFR2 but not VEGFR3 governs integrity and remodeling of thyroid angiofollicular unit in normal state and during goitrogenesis EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28438786] (IHC,, Mouse) IHC Mouse
S Yazdani, F Poosti, L Toro, J Wedel, R Mencke, K Mirkovi?, MH de Borst, JS Alexander, G Navis, H van Goor, J van den Bo, JL Hillebrand Vitamin D inhibits lymphangiogenesis through VDR-dependent mechanisms Sci Rep, 2017;7(0):44403. 2017 [PMID: 28303937] (ICC,, Mouse) ICC Mouse
Show All 49 Publications.

Reviews for VEGF R3/Flt-4 Antibody (AF743) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF743:
Filter by Applications
WB
(1)
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/09/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19901262
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19901262

Details

Detection NotesSee PMID 19901262

Comments

CommentsPublished: See PMID 19901262Independent review from antibodyresource.com
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23878309
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 19901262
SpeciesMouse

Blocking

Blocking DetailsSee PMID 19901262

Details

Detection NotesSee PMID 19901262

Comments

Commentspublished: See PMID 19901262Independent review from antibodyresource.com

Product General Protocols

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FAQs for VEGF R3/Flt-4 Antibody (AF743) (0)

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Secondary Antibodies

 

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Blogs on VEGF R3/Flt-4. Showing 1-10 of 49 blog posts - Show all blog posts.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

Showing 1-10 of 49 blog posts - Show all blog posts.

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
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Anonymous
01/09/2015
Application: WB
Species: Mouse

 
Anonymous
01/09/2015
Application: IHC-Fr
Species: Mouse

 
Anonymous
01/09/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol FLT4
Entrez
Uniprot