VEGF R3/Flt-4 Antibody (AFL4)


Flow Cytometry: VEGF R3/Flt-4 Antibody (AFL4) [NBP1-43259] - Surface staining of MC/9 cell line with Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (left) and PE (right). Appropriate isotype controls were more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-Fr, IP
0.5 mg/ml

VEGF R3/Flt-4 Antibody (AFL4) Summary

The immunogen for this antibody was VEGFR3.
IgG2a Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous, may contain carrier protein/stabilizer.
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The AFL4 antibody has been tested by flow cytometric analysis of in vitro differentiated mouse endothelial cells. In brief, mouse ES cells were incubated on collagen IV matrix for 4 days and subsequently stimulated with VEGF under serum free conditions to induce further differentiation.

Reactivity Notes

This antibody is reactive to Mouse.

Alternate Names for VEGF R3/Flt-4 Antibody (AFL4)

  • EC 2.7.10
  • EC
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4


The AFL4 monoclonal antibody reacts with the mouse VEGF receptor-3, also known as Flt-4. This 195 kDa molecule was identified as an endothelial-specific member of the receptor tyrosine kinase (RTK) family. During early embryogenesis all endothelial cells express VEGFR-3, while in the adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. However, VEGFR-3 expression is induced in the adult tissue upon tumor implementation suggesting an important role for this receptor in the tumor angiogenesis. VEGF-C and VEGF-D bind to and activate VEGFR-3. AFL4 is an antagonist mAb.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-43259AF405
Alexa Fluor (R) 488 Labeled NBP1-43259AF488
Alexa Fluor (R) 647 Labeled NBP1-43259AF647
Alexa Fluor (R) 700 Labeled NBP1-43259AF700
Allophycocyanin Labeled NBP1-43259APC
Biotin Labeled NBP1-43502
DyLight 350 Labeled NBP1-43259UV
DyLight 405 Labeled NBP1-43259V
DyLight 405LS Labeled NBP1-43259V3
DyLight 488 Labeled NBP1-43259G
DyLight 680 Labeled NBP1-43259FR
DyLight 755 Labeled NBP1-43259IR
PE Labeled NBP1-43259PE
PerCP Labeled NBP1-43259PCP

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Contact Information

Product PDFs


Gene Symbol FLT4

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