VEGF R3/Flt-4 Antibody (9D9)

Images

 
Western Blot: VEGF R3/Flt-4 Antibody (9D9) [NBP1-18651] - Analysis using recombinant human soluble VEGF Receptor 3 as target.
Immunocytochemistry/ Immunofluorescence: VEGF R3/Flt-4 Antibody (9D9) [NBP1-18651] - Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in immortalized lymphatic endothelial cells (LEC-Tert). Left panel negative ...read more
Flow Cytometry: VEGF R3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal lymphatic endothelial cells (HDLEC).
Immunocytochemistry/ Immunofluorescence: VEGF R3/Flt-4 Antibody (9D9) [NBP1-18651] - Immunofluorescence staining of human VEGFR-3/FLT-4 (red) in hFlt4-transfected MG63 cells. Monoclonal mouse anti-human FLT-4 #9D9 and ...read more
Flow Cytometry: VEGF R3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
9D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

VEGF R3/Flt-4 Antibody (9D9) Summary

Immunogen
Recombinant human soluble FLT-4 protein
Specificity
Mouse Monoclonal to VEGFR-3 (#1)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-5ug/ml
  • ELISA 1-5ug/ml
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-10ug/ml
  • Immunohistochemistry 1-10ug/ml
  • CyTOF-ready
Application Notes
ELISA: To detect human VEGFR-3/FLT-4 by direct ELISA a concentration of 0.5 - 1.5 ug/ml can be used. This purified IgG, in combination with compatible secondary reagents, allows the detection of 0.25- 0.5 ng/well rhVEGFR-3/FLT-4. For Western blot analysis, the antibody can be used at 0.5 - 1 ug/ml with the appropriate secondary reagents to detect human VEGFR-3/FLT-4. Depending on the visualisation method the detection limit for rh VEGFR-3/FLT-4 is approximately 5 ng/lane under reducing conditions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 50 ul sterilized water to desired concetration.

Notes

Clone #1 changed to 9D9 as a more unique identifier.

Alternate Names for VEGF R3/Flt-4 Antibody (9D9)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Biotin Labeled NBP1-18652

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Bioinformatics

Gene Symbol FLT4
Entrez