VEGF R3/Flt-4 Antibody (2E11)

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ELISA: VEGF R3/Flt-4 Antibody (2E11) [NBP1-18654] - VEGFR-3/FLT4 Sandwich-ELISA using recombinant human soluble FLT-4 as standard. Mouse anti-human VEGFR-3/FLT4 was used as capture antibody, Biotinylated rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

VEGF R3/Flt-4 Antibody (2E11) Summary

Immunogen
Recombinant human soluble FLT-4 protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 0.5-1.5 ug/ml
  • Immunoprecipitation 1-5 ug/ml
Application Notes
ELISA: To detect human VEGFR-3/FLT-4 by direct ELISA a concentration of 0.5 - 1.5 ug/ml can be used. This purified IgG, in combination with compatible secondary reagents, allows the detection of 0.25- 0.5 ng/well rhVEGFR-3/FLT-4. Western Blot: For Western blot analysis, the antibody can be used at 0.5 - 1 ug/ml with the appropriate secondary reagents to detect human VEGFR-3/FLT-4. Depending on the visualisation method the detection limit for rh VEGFR-3/FLT-4 is approximately 5 ng/lane under reducing conditions. Immunohistochemistry: Use at 1-10 ug/ml

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 50 ul sterilized water to desired concetration.

Notes

Clone #2 changed to 2E11 as a more unique identifier.

Alternate Names for VEGF R3/Flt-4 Antibody (2E11)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

FAQs for VEGF R3/Flt-4 Antibody (NBP1-18654). (Showing 1 - 1 of 1 FAQ).

  1. We are interested to detect a soluble form of FLT4 (VEGF-receptor 3) in human plasma samples. Using an ELISA-experiment, we got a signal and now we are interested to identify the exact region (protein sequence) of this soluble form. For this propose, we plan an IP followed by mass spectroscopy. We found in your catalog that the antibody NBP1-18654 can be used by IP. Can you please confirm that your antibody will detect the soluble form of FLT4 (AS1-AS1276)? Can we use your protocol for IP published on your home page or should we use a different protocol? Is our choice from all your FLT4 antibodies ok or would you recommend another antibody for our propose?
    • NBP1-18654 does bind to soluble FLT4. This antibody should work with any general IP protocol.

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Bioinformatics

Gene Symbol FLT4
Entrez
Uniprot