Human VEGF R2/KDR/Flk-1 ELISA Kit (Colorimetric)

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ELISA: VEGF R2/KDR/Flk-1 ELISA Kit [NBP1-83729] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
78 - 5000 pg/mL
Sensitivity
7.0 pg/mL

Order Details

Human VEGF R2/KDR/Flk-1 ELISA Kit (Colorimetric) Summary

Standard Curve Range
78 - 5000 pg/mL
Sensitivity
7.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
9.8%
Intra-Assay
5.3%
Spike Recovery
Refer to manual
Sample Volume
50 uL (prediluted 1:25)
Kit Type
ELISA Kit (Colorimetric)
Gene
KDR

Applications/Dilutions

Application Notes
The human VEGF-R2/KDR ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-R2/KDR.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human VEGF-R2/KDR
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Human VEGF-R2/KDR Standard lyophilized, 10 ng/mL upon reconstitution
  5. Biotin-Conjugate anti-human VEGF-R2/KDR monoclonal antibody
  6. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  7. Streptavidin-HRP
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)

Alternate Names for Human VEGF R2/KDR/Flk-1 ELISA Kit (Colorimetric)

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics Tool for VEGF R2/KDR/Flk-1 ELISA Kit (NBP1-83729)

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Blogs on VEGF R2/KDR/Flk-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

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Bioinformatics

Gene Symbol KDR
Entrez