VEGF R2/KDR/Flk-1 Antibody


Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Detection of VEGFR2 in CSF-VEGF R2/KDR/Flk-1 transfected lysates using NB 100-627. ECL detection in 15 seconds. Lane 1 - 1:2,000 Lane 2 - 1:10,000.
Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Analysis of VEGF R2/KDR/Flk-1 in mouse brain tumor lysates using anti-VEGF R2/KDR/Flk-1 antibody. Image from verified customer review.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

VEGF R2/KDR/Flk-1 Antibody Summary

Residues corresponding to the kinase insert or C-terminus of mouse VEGF Receptor 2 (residues 1158-1365). [Swiss-Prot# P35918]
Endothelial Cell Marker
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
This VEGF Receptor 2 antibody is useful for Immunohistochemistry paraffin-embedded sections (PMID 10751359), Immunoprecipitation and Western blot where a doublet can been seen at ~ 150 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
150 kDa
Reviewed Applications
Read 1 Review rated 5
NB100-627 in the following application:

Read Publications using
NB100-627 in the following applications:

  • 1 publication
  • IP
    3 publications
  • WB
    6 publications

Reactivity Notes

Human and mouse.

Alternate Names for VEGF R2/KDR/Flk-1 Antibody

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGFR2
  • VEGFR-2


VEGFs such as VEGFA, VEGFC, VEGFD etc. activate RTKs namely VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4), that regulates angiogenic as well as lymphangiogenic transduction signals. VEGF Receptor 2 (VEGFR2) is the most prominent cell-surface receptor for VEGF ligands and binding of VEGFs to isoform 1 leads to multiple signaling cascades activation. PLCG1 activation results in production of signaling molecules (diacylglycerol, inositol 1,4,5-trisphosphate etc) and PKC activation. VEGFR2 is involved in the activation of MAPK1/ERK2, MAPK3/ERK1, and MAPK as well as AKT signaling pathways. It facilitates phosphorylation of PIK3R1 (regulatory subunit of PI3K), cytoskeletal actin reorganization and PTK2/FAK1 activation. VEGFR2 is essential for VEGFA-mediated induction of NOS2/NOS3, leading to the production of NO by endothelial cells and is involved in phosphorylation of FYN, NCK1, NOS3, PIK3R1 and SRC. Isoforms -2 and -3 function as decoy receptors for VEGFs, and isoform -2 plays as negative regulator of VEGFA/VEGFC-mediated lymphangiogenesis by limiting free VEGFA and/or VEGFC levels alongwith preventing their binding to VEGFR3. Versatile signaling effects by VEGFR2 leads to its regulatory role in processes such as angiogenesis, vascular development, vascular permeability, embryonic hematopoiesis, and promotes proliferation, survival, migration and differentiation of endothelial cells alongwith facilitating reorganization of the actin cytoskeleton.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu
Applications: WB, ChIP, ELISA
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC
Species: Mu, Rt
Applications: WB, Flow, IHC, Block
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu, Mu
Applications: WB, Simple Western, ICC

Publications for VEGF R2/KDR/Flk-1 Antibody (NB100-627)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-P, IP, WB.

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Showing Publications 1 - 8 of 8.
Publications using NB100-627 Applications Species
Ying R, Wang XQ, Yang Y et al. Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway. Life Sci. 2016 Jan 01 [PMID:26656263] (WB, Human) WB Human
Andre H, Tunik S, Aronsson M, Kvanta A. Hypoxia-Inducible Factor-1alpha Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model. ARVO Journals. 2015 Oct [PMID:26501235] (WB, Mouse) WB Mouse
Tsuboi I, Yamashita T, Nagano M et al. Impaired expression of HIF -2alpha induces compensatory expression of HIF-1alpha for the recovery from anemia J. Cell. Physiol. 2015 Jan 03 [PMID:25557133] (WB, Mouse) WB Mouse
Puglisi F, Puppin C, Pegolo E et al. Expression of periostin in human breast cancer. J Clin Pathol;614:494-8. 2008 Apr. [PMID:17938160]
Singh, AJ et al. The carboxyl terminus of VEGFR-2 is required for PKC-mediated down-regulation. Mol Biol Cell;164:2106-18. 2005 Apr. [PMID:15673613] (WB, IP, Mouse) WB, IP Mouse
Meyer, RD et al. The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling. J Biol Chem;2791:735-42. 2004 Jan 2. [PMID:14573614] (WB, IP) WB, IP
Lashkari K, Hirose T, Yazdany J, McMeel JW, Kazlauskas A, Rahimi N. Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity. Am J Pathol;1564:1337-44. 2000 Apr. [PMID:10751359] (IHC-P, Human) IHC-P Human
Rahimi, N et al. Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 VEGFR-1 modulates mitogenic activity of VEGFR-2 in endothelial cells. J Biol Chem;27522:16986-92. 2000 Jun 2. [PMID:10747927] (WB, IP, Mouse) WB, IP Mouse

Reviews for VEGF R2/KDR/Flk-1 Antibody (NB100-627) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-627:
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Images Ratings Applications Species Date Details
Western Blot VEGF R2/KDR/Flk-1 NB100-627
reviewed by:
WB Mouse 12/12/2014


ApplicationWestern Blot
Sample TestedGL261 implanted mouse glioma tissue


Blocking Details5% BSA+TBST, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:5000, overnight, 4 degrees Celsius, 5% BSA+TBST

Secondary Antibody

Secondary DescriptionGoat anti-rabbit, HRP
Secondary Manufacturer Cat#656120 (Life Technologies)
Secondary Concentration1:3000 in TBST+5% BSA


Detection NotesECL Chemiluminescent Kit, UVP, 30 second exposure time

Product General Protocols

View specific protocols for VEGF R2/KDR/Flk-1 Antibody (NB100-627): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VEGF R2/KDR/Flk-1 Antibody (NB100-627) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on VEGF R2/KDR/Flk-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol KDR

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