VEGF R2/KDR/Flk-1 Antibody

Images

 
Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet inVEGF R2/KDR/Flk-1 induced HUVEC lysate (50 ug) using NB100-529 (4 ug/ml). ECL detection 2.5 minutes.
Immunocytochemistry/ Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - The VEGF R2/KDR/Flk-1antibody was tested in V6.5 mouse embryonic stem cells (Catalog No. NBP1-41162) against Dylight 488 (Green). ...read more
Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-529] - Detection of VEGF R2/KDR/Flk-1 doublet in CSF-1 receptor/VEGF Receptor 2 chimera transfected lysate (20 ug) using NB100-529 (6 ug/ml). ECL detection 30 seconds.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.6 mg/ml

VEGF R2/KDR/Flk-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the mouse VEGF Receptor 2 protein (between residues 1200-1300). [Swiss-Prot# P35918]
Localization
Type 1 membrane protein
Marker
Endothelial Cell Marker
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
Application Notes
This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa. In ICC/IF membrane staining can be seen in V6.5 mouse embryonic stem cells (cat# NBP1-41162). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
VEGF R2/KDR/Flk-1 Lysate (NBL1-12223)
v6.5 Mouse embryonic stem cells (NBP1-41162)

Reactivity Notes

Immunogen sequence has 100% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
2.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VEGF R2/KDR/Flk-1 Antibody

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFs such as VEGFA, VEGFC, VEGFD etc. activate RTKs namely VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), and VEGFR-3 (Flt-4), that regulates angiogenic as well as lymphangiogenic transduction signals. VEGF Receptor 2 (VEGFR2) is the most prominent cell-surface receptor for VEGF ligands and binding of VEGFs to isoform 1 leads to multiple signaling cascades activation. PLCG1 activation results in production of signaling molecules (diacylglycerol, inositol 1,4,5-trisphosphate etc) and PKC activation. VEGFR2 is involved in the activation of MAPK1/ERK2, MAPK3/ERK1, and MAPK as well as AKT signaling pathways. It facilitates phosphorylation of PIK3R1 (regulatory subunit of PI3K), cytoskeletal actin reorganization and PTK2/FAK1 activation. VEGFR2 is essential for VEGFA-mediated induction of NOS2/NOS3, leading to the production of NO by endothelial cells and is involved in phosphorylation of FYN, NCK1, NOS3, PIK3R1 and SRC. Isoforms -2 and -3 function as decoy receptors for VEGFs, and isoform -2 plays as negative regulator of VEGFA/VEGFC-mediated lymphangiogenesis by limiting free VEGFA and/or VEGFC levels alongwith preventing their binding to VEGFR3. Versatile signaling effects by VEGFR2 leads to its regulatory role in processes such as angiogenesis, vascular development, vascular permeability, embryonic hematopoiesis, and promotes proliferation, survival, migration and differentiation of endothelial cells alongwith facilitating reorganization of the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for VEGF R2/KDR/Flk-1 Antibody (NB100-529): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VEGF R2/KDR/Flk-1 Antibody (NB100-529) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-529AF405
Alexa Fluor 488 Labeled NB100-529AF488
Alexa Fluor 647 Labeled NB100-529AF647
Alexa Fluor 700 Labeled NB100-529AF700
Biotin Labeled NB100-529B
DyLight 405 Labeled NB100-529V
DyLight 488 Labeled NB100-529G
DyLight 550 Labeled NB100-529R
DyLight 650 Labeled NB100-529C
FITC Labeled NB100-529F
HRP Labeled NB100-529H

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Blogs on VEGF R2/KDR/Flk-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

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Bioinformatics

Gene Symbol KDR
Entrez