A synthetic peptide made to a C-terminal region of the mouse VEGF Receptor 2 protein (between residues 1300-1367). [Swiss-Prot# P35918]
Type I membrane protein
Endothelial Cell Marker
This has only been shown to recognize the native form (180-190 kDa protein) of VEGFR-2, known as Flk-1 / KDR / VEGFR 2 (Vascular Endothelial Growth Factor Receptor 2), though it was raised against the precursor form.
Immunogen affinity purified
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Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified
Western Blot 1 ug/ml
Flow Cytometry 1:100
This VEGF Receptor 2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a doublet is seen at ~150 kDa with CSF-1R/VEGFR2 chimera transfected lysates representing VEGFR-2. A non-specific band is also seen at ~85 kDa. In ICC/IF punctate membrane staining was observed in Hela cells.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NB100-2382G in the following applications:
Human. Predicted to react with mouse based on 100% sequence homology. Immunogen sequence has 79% homology to rat. Hamster reactivity reported in scientific literature (PMID: 23763710)
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for VEGF R2/KDR/Flk-1 Antibody [DyLight 488]
Fetal liver kinase 1
fetal liver kinase-1
FLK1tyrosine kinase growth factor receptor
kinase insert domain receptor (a type III receptor tyrosine kinase)
Kinase insert domain receptor
Protein-tyrosine kinase receptor flk-1
vascular endothelial growth factor receptor 2
Receptors for VEGF, VEGFB and PGF have a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for VEGF R2/KDR/Flk-1 Antibody (NB100-2382G). (Showing 1 - 2 of 2 FAQ).
I am interested in NB100-2382G and would like to purchase isotype control for this. Could you tell me what the isotype is?
Since NB100-2382G is a polyclonal antibody, it does not have one particular isotype. As for this antibody, you can suggest our rabbit-IgG isotype control, however this product is un-conjugated but if you are interested in purchasing it, we can provide the conjugated control as well. Here is more information on this product.
We recently ordered NB100-2382G, and are searching for an appropriate isotype control antibody. We are considering NB810-56910, but this product is not conjugated to DyLight 488, as is NB100-2382G. Are there any Rabbit IgG isotype controls that are conjugated to DyLight 488? Do you recommend any other antibodies for this purpose? Thanks!
We would be happy to custom conjugate catalog number NB810-56910 to DyLight 488.
VEGFR-2 - A highly active kinase VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry... Read full blog post.