VEGF R2/KDR/Flk-1 Antibody

Images

 
Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164 (Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner ...read more
VEGF R2/KDR/Flk‑1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Mouse VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

VEGF R2/KDR/Flk-1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2
Ala20-Glu762
Accession # P35918
Specificity
Detects mouse VEGF R2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VEGF R2 is observed and less than 2% cross-reactivity with recombinant mouse (rm) VEGF R1 and rmVEGF R3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KDR
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Neutralization 0.1-0.3 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF644 in the following applications:

Publications
Read Publications using
AF644 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF R2/KDR/Flk-1 Antibody

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGF R2/KDR/Flk-1 Antibody (AF644)(37)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 13 applications: Bioassay, ELISA Development, Flow, ICC, IHC, IHC Fresh, IHC Frozen, IHC Not Specified, IHC Paraffin-embedded, IP, In vivo, Neut, WB.


Filter By Application
Bioassay
(1)
ELISA Development
(2)
Flow
(1)
ICC
(3)
IHC
(5)
IHC Fresh
(3)
IHC Frozen
(1)
IHC Not Specified
(3)
IHC Paraffin-embedded
(3)
IP
(3)
In vivo
(5)
Neut
(1)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(28)
Rat
(4)
Canine
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using AF644 Applications Species
J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh Impaired angiopoietin/Tie2 signaling compromises Schlemm&#039;s canal integrity and induces glaucoma J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28920924] (IHC,, Mouse) IHC Mouse
H Wu, J Zhao, B Fu, S Yin, C Song, J Zhang, S Zhao, Y Zhang Retinoic acid-induced upregulation of miR-219 promotes the differentiation of embryonic stem cells into neural cells Cell Death Dis, 2017;8(7):e2953. 2017 [PMID: 28749472] (WB,, Mouse) WB Mouse
UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017;19(3):189-201. 2017 [PMID: 28218908] (IHC,, Mouse) IHC Mouse
MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins Genesis, 2016;0(0):. 2016 [PMID: 27060598] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation. J Clin Invest, 2015;125(7):2661-76. 2015 [PMID: 26053665] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 2015;21(5):483-91. 2015 [PMID: 25894826] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Hou, Sheng Ta, Nilchi, Ladan, Li, Xuesheng, Gangaraju, Sandhya, Jiang, Susan X, Aylsworth, Amy, Monette, Robert, Slinn, Jacqueli Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage. Sci Rep, 2015;5(0):7890. 2015 [PMID: 25601765] (Bioassay,, Rat) Bioassay Rat
Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K The Schlemm&#039;s canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel. J Clin Invest, 2014;124(9):3975-86. 2014 [PMID: 25061878] (IHC Fresh,, Mouse) IHC Fresh Mouse
Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma. Cancer Res, 2013;73(24):7254-64. 2013 [PMID: 24158094] (WB,, Human) WB Human
Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton D, Bissig K, Kalir T, Jacobs A, Evans T, Evans M, Gouon-Evans V KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development. Cell Stem Cell, 2013;12(6):748-60. 2013 [PMID: 23746980] (IHC Fresh,, Mouse) IHC Fresh Mouse
Show All 37 Publications.

Reviews for VEGF R2/KDR/Flk-1 Antibody (AF644) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: IHC-Fr, IF.

Reviews using AF644:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23746980
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 01/09/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23746980
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23746980

Details

Detection NotesSee PMID 23746980

Comments

CommentsPublished: See PMID 23746980Independent review from antibodyresource.com

Product General Protocols

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FAQs for VEGF R2/KDR/Flk-1 Antibody (AF644) (0)

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Diseases for VEGF R2/KDR/Flk-1 Antibody (AF644)

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Blogs on VEGF R2/KDR/Flk-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
01/09/2015
Application: IHC-Fr
Species: Mouse

 
Anonymous
01/09/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol KDR
Entrez
Uniprot