VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66)

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Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66) [NB110-60967] - Human placenta.
Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66) [NB110-60967] - Normal mouse kidney section was subjected to immunohistochemistry staining (ABC) of VEGF R2/KDR/Flk-1 using NB110-60967. Specific ...read more
Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66) [NB110-60967] - Human placenta.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB110-60967
    • Availability
      Product Discontinued

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VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human VEGFR2 N-terminal recombinant protein.
Marker
Endothelial Cell Marker
Specificity
VEGFR2. This antibody shows no cross-reactivity with hVEGFR1, hVEGFR3.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDR
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:400-1:1000
  • ELISA 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
Application Notes
In western blots, this antibody shows no cross-reactivity with hVEGFR1, hVEGFR3.
Positive Control
VEGF R2/KDR/Flk-1 Lysate (NBL1-12223)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for VEGF R2/KDR/Flk-1 Antibody (MM0002-2F66)

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for VEGF R2/KDR/Flk-1 Antibody (NB110-60967). (Showing 1 - 1 of 1 FAQ).

  1. I'm interested in a mouse MAb against VEGFR2 for IHC on FFPE human tumors but I was wondering how clean the western blot is for NB110-60967. Is the Ab pretty specific or are there additional bands that may provide non-specific brown stuff by IHC?
    • NB110-60967 is suitable for IHC-Paraffin on human tissues. In Western blot, this antibody shows no cross-reactivity with hVEGFR1, hVEGFR3, hence no presence of extra bands can be seen.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional VEGF R2/KDR/Flk-1 Products

Bioinformatics Tool for VEGF R2/KDR/Flk-1 Antibody (NB110-60967)

Discover related pathways, diseases and genes to VEGF R2/KDR/Flk-1 Antibody (NB110-60967). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for VEGF R2/KDR/Flk-1 Antibody (NB110-60967)

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Pathways for VEGF R2/KDR/Flk-1 Antibody (NB110-60967)

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PTMs for VEGF R2/KDR/Flk-1 Antibody (NB110-60967)

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Research Areas for VEGF R2/KDR/Flk-1 Antibody (NB110-60967)

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Blogs on VEGF R2/KDR/Flk-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

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Bioinformatics

Gene Symbol KDR
Entrez
Uniprot