VEGF R2/KDR/Flk-1 Antibody (4B4)

Images

 
Western Blot: VEGF R2/KDR/Flk-1 Antibody (4B4) [NBP1-51581] - Western blot analysis using KDR mouse mAb against extracellular domain of human KDR (aa20-764).
Immunocytochemistry/ Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (4B4) [NBP1-51581] - Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using KDR mouse mAb (green). Blue: DRAQ5 fluorescent ...read more
Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (4B4) [NBP1-51581] - Flow cytometric analysis of HepG2 cells using KDR mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
4B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

VEGF R2/KDR/Flk-1 Antibody (4B4) Summary

Immunogen
Purified recombinant extracellular fragment of human KDR (aa20-764) fused with hIgGFc tag expressed in HEK293 cells.
Marker
Endothelial Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDR
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Theoretical MW
152 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for VEGF R2/KDR/Flk-1 Antibody (4B4)

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

Chimera RNA interference (chimera RNAi) is process by which small interfering RNA/DNA chimera triggers the destruction of mRNA for the original gene. The discovery work, design, and application of chimera RNAi has been pioneered by Professor Kaoru Saigo and Dr. Kumiko Ui-Tei at the University of Tokyo. Chimera RNAi has many advantages over the conventional siRNAs. First, it has been demonstrated to have reliable knock-down for over 10,000 human genes. Because the human genome is composed of an intricate, genetic network, chimera RNAi's unique design has successfully obviated the off-target effects including microRNA-based influence. Another advantage of the chimera RNAi technology is its effectiveness at low concentrations (0.5nM to 5nM); only mRNA is destroyed so genomic genes are not affected. Finally, having both the sense and anti-sense strands consisting RNA/DNA chimera, it offers much greater compound stability for streamlining in vitro and in vivo assays and applications while minimizing interferon induction and other adverse reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGF R2/KDR/Flk-1 Antibody (NBP1-51581) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Additional VEGF R2/KDR/Flk-1 Products

Bioinformatics Tool for VEGF R2/KDR/Flk-1 Antibody (NBP1-51581)

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Diseases for VEGF R2/KDR/Flk-1 Antibody (NBP1-51581)

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Pathways for VEGF R2/KDR/Flk-1 Antibody (NBP1-51581)

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PTMs for VEGF R2/KDR/Flk-1 Antibody (NBP1-51581)

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Blogs on VEGF R2/KDR/Flk-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

VEGFR-2 - A highly active kinase
VEGFR-2 is a family member of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. It is a key regulator of the process of angiogenesis that takes place during fundamental developmental processes such as embry...  Read full blog post.

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Bioinformatics

Gene Symbol KDR
Entrez