Recombinant VEGF Protein Summary
A partial protein corresponding to VEGFA.
E. coli expressed amino acids ala 27-arg 147 of mature human VEGF121 (accession # AF214570).
|Details of Functionality
Measured by human umbilical vein endothelial cell proliferation assay. The ED50 is typically 10.0 ng/ml, corresponding to a specific activity of 1.0 x 105 Units/mg.
| Protein/Peptide Type
Partial Recombinant Protein
Less than 0.01 ng/ug cytokine as determined by the LAL assay.
This recombinant human VEGF has been tested by bioassay of human umbilical vein endothelial cells (HUVEC). Dose-dependent induction of proliferation of HUVECs yields an ED50 of 10.0 ng/ml, corresponding to a specific activity of approximately 1x10^5Units/mg.
This protein was made to the human sequence.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Sterile liquid; 35% (v/v) acetonitrile, 0.1% (v/v) trifluoracetic acid with 1.0% BSA as a stabilizer. 0.22 uM filtered.
0.09% Sodium Azide
Purity: Greater than 98% as determined by SDS-PAGE
Alternate Names for Recombinant VEGF Protein
- vascular endothelial growth factor A
- Vascular permeability factor
- VPFvascular endothelial growth factor
Vascular endothelial growth factor (VEGF), also called vascular permeability factor (VPF), is produced by cultured vascular smooth muscle cells. From analysis of transcripts from these cells by PCR and cDNA cloning, 3 different forms of the VEGF coding region have been identified. These cDNAs had predicted products of 189, 165, and 121 amino acids. VEGF is a mitogen primarily for vascular endothelial cells. It is structurally related to platelet-derived growth factor. VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The VEGF receptor, Flk1, is exclusively expressed in endothelial cells. VEGF may be a major regulator of tumor angiogenesis in vivo.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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Blogs on VEGF. Showing 1-10 of 16 blog posts - Show all blog posts.
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The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
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Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel... Read full blog post.
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Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits.... Read full blog post.
HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a... Read full blog post.
HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy... Read full blog post.
VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g... Read full blog post.