VEGF Recombinant Protein


SDS-Page: VEGF Protein [NBC1-21277] - Human Recombinant E.coli protein

Product Details

Reactivity HuSpecies Glossary
Applications PAGE
1.0 mg/ml

Order Details

VEGF Recombinant Protein Summary

A recombinant protein corresponding to amino acids 207 - 327 of VEGFA.

Source: E. coli


Details of Functionality
The ED50 for this effect is <1.8ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
20 mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol
No Preservative
1.0 mg/ml


Application Notes
Cell Line: NIH-3T3 (Mus mosculus/ embryo)
Maintenance Condition: RPMI 1640 containing 10% FBS
Assay Medium: serum free RPMI 1640
Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul)
Serum Free Starvation: 24hr with RPMI1640
Incubation Time: 24 hr (after sample treatment)
Concentration Range: 100 pg/ml - 10 ug/ml
Detection method: BrdU assay
Theoretical MW
14.2 kDa


The purity of this protein is > 95% by SDS PAGE. Molecular weight is 14.2 kDa (122aa), confirmed by MALDI-TOF.

Alternate Names for VEGF Recombinant Protein

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for VEGF Protein (NBC1-21277). (Showing 1 - 1 of 1 FAQ).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Blogs on VEGF. Showing 1-10 of 12 blog posts - Show all blog posts.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.

LYVE1 - It's Alive! It's a LYVE
LYVE1 (lymphatic vessel endothelial hyaluronan receptor-1) is one of the most specific and widely used mammalian lymphatic endothelial markers.It is found in lymph nodes and at the luminal/abluminal surfaces of lymphatic vessels. It is a single-pass t...  Read full blog post.

LOX: A prime enzyme
LOX is a copper-dependent amine oxidase enzyme that executes post-translational oxidative deamination on peptidyl lysine residues in precursors of fibrous collagen and elastin. LOX is secreted into the extracellular environment in an inactive form, wh...  Read full blog post.

VEGF Receptors, Angiogenesis and Cancer
Vascular endothelial growth factor receptors 1 and 2 (VEGFR1 and VEGFR2) are related family members of the vascular endothelial growth factor (VEGF) family of membrane receptor tyrosine kinases. They are key regulators of physiological angiogenesis du...  Read full blog post.

Controlling the HIF-1 Switch
Hypoxia-inducible factor-1 is a major transcription factor composed of two subunits: HIF-1alpha and HIF-1 beta. Under normoxic conditions, HIF-1 alpha is targeted to proteosomal degradation via ubiquitination. On the other hand during hypoxic conditio...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

Contact Information

Product PDFs


Gene Symbol VEGFA

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