Human VEGF ELISA Kit (Colorimetric)


ELISA: VEGF ELISA Kit [NBP1-91272] - Standard Curve

Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
15.6 - 1,000 pg/mL
7.9 pg/mL

Order Details

Human VEGF ELISA Kit (Colorimetric) Summary

The assay detects both natural and recombinant human VEGF-A. Crossreactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-A positive serum. There was no crossreactivity detected, notably not with human VEGF-B, VEGF-C, VEGF-D and PfGF. Interference was detected for VEGF-R1 at concentrations > 200 pg/ml, and not for VEGF-R2.
Standard Curve Range
15.6 - 1,000 pg/mL
7.9 pg/mL
Assay Type
Sandwich ELISA
Spike Recovery
Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)


Application Notes
The human VEGF-A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-A

Reactivity Notes


Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. Substrate Solution (tetramethyl-benzidine)
  2. Streptavidin-HRP
  3. Stop Solution (1M Phosphoric acid)
  4. Sample Diluent
  5. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  6. Human VEGF-A Standard lyophilized, 2 ng/mL upon reconstitution
  7. Biotin-Conjugate anti-human VEGF-A polyclonal antibody
  8. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  9. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human VEGF-A
  10. Adhesive Films

Alternate Names for Human VEGF ELISA Kit (Colorimetric)

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor


One of the key molecules for angiogenesis and for the survival of the endothelium is vascular endothelial growth factor (VEGF-A). It is a specific endothelial cell mitogen and a strong vascular permeability factor (VPF). VEGF-A is a heparin-binding glycoprotein, secreted as a homodimer of 45 kDa by many different cell types. VEGF-A also causes vasodilation through the nitiric oxide synthase pathway in endothelial cells and can activate migration in monocytes. Many different splice variants of VEGF-A have been described, but VEGF165 is the most predominant protein and anchors with its heparin binding domain to extracellular matrix and to heparin sulfate. Examples where VEGF-A plays an important role are psoriasis andrheumatoid arthritis, as well as the ovarian hyperstimulation syndrome. Diabetic retinopathy is associated with high intraocular levels of VEGF-A, and inhibition of VEGF-A function may result in infertility by blockage of corpus luteum function. Direct demonstration of the importance of VEGF-A in tumor growth has been achieved using dominant negative VEGF receptors to block in vivo proliferation, as well as blocking antibodies to VEGF or to one of the VEGF receptors. VEGF-A transcription is highly activated by hypoxia and by oncogenes like H-ras and several transmembrane tyrosine kinases, such as epidermal growth factor receptor and ErbB2. Together these pathways account for a marked upregulation of VEGF-A in tumors compared to normal tissues and are often of prognostic importance and relevance. Targeting the VEGF signalling may be of major therapeutic importance for many diseases and serves as a basis for the design of future (anti)-angiogenic treatments.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for VEGF ELISA Kit (NBP1-91272). (Showing 1 - 1 of 1 FAQ).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
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The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
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Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
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Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

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Gene Symbol VEGFA