Human VEGF ELISA Kit (Colorimetric) Summary
The assay detects both natural and recombinant human VEGF-A. Crossreactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-A positive serum. There was no crossreactivity detected, notably not with human VEGF-B, VEGF-C, VEGF-D and PfGF. Interference was detected for VEGF-R1 at concentrations > 200 pg/ml, and not for VEGF-R2.
|Standard Curve Range
15.6 - 1,000 pg/mL
Please see protocol for more specific Spike Recovery details.
ELISA Kit (Colorimetric)
The human VEGF-A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-A
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Stop Solution (1M Phosphoric acid)
- Sample Diluent
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Human VEGF-A Standard lyophilized, 2 ng/mL upon reconstitution
- Biotin-Conjugate anti-human VEGF-A polyclonal antibody
- Adhesive Films
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human VEGF-A
Alternate Names for Human VEGF ELISA Kit (Colorimetric)
- vascular endothelial growth factor A
- Vascular permeability factor
- VPFvascular endothelial growth factor
One of the key molecules for angiogenesis and for the survival of the endothelium is vascular endothelial growth factor (VEGF-A). It is a specific endothelial cell mitogen and a strong vascular permeability factor (VPF). VEGF-A is a heparin-binding glycoprotein, secreted as a homodimer of 45 kDa by many different cell types. VEGF-A also causes vasodilation through the nitiric oxide synthase pathway in endothelial cells and can activate migration in monocytes. Many different splice variants of VEGF-A have been described, but VEGF165 is the most predominant protein and anchors with its heparin binding domain to extracellular matrix and to heparin sulfate. Examples where VEGF-A plays an important role are psoriasis andrheumatoid arthritis, as well as the ovarian hyperstimulation syndrome. Diabetic retinopathy is associated with high intraocular levels of VEGF-A, and inhibition of VEGF-A function may result in infertility by blockage of corpus luteum function. Direct demonstration of the importance of VEGF-A in tumor growth has been achieved using dominant negative VEGF receptors to block in vivo proliferation, as well as blocking antibodies to VEGF or to one of the VEGF receptors. VEGF-A transcription is highly activated by hypoxia and by oncogenes like H-ras and several transmembrane tyrosine kinases, such as epidermal growth factor receptor and ErbB2. Together these pathways account for a marked upregulation of VEGF-A in tumors compared to normal tissues and are often of prognostic importance and relevance. Targeting the VEGF signalling may be of major therapeutic importance for many diseases and serves as a basis for the design of future (anti)-angiogenic treatments.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for VEGF ELISA Kit (NBP1-91272). (Showing 1 - 1 of 1 FAQ).
Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
- I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
Additional Array Products
Bioinformatics Tool for VEGF ELISA Kit (NBP1-91272)
Discover related pathways, diseases and genes to VEGF ELISA Kit (NBP1-91272). Need help?
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