VEGF-B Recombinant Protein Summary
A recombinant protein with a His-tag corresponding to amino acids 22 to 207 of Human VEGF-B
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
|Details of Functionality
Measured in a cell proliferation assay using NIH-3T3 mouse
embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast )
2. Maintenance Condition: RPMI 1640 containing 10% FBS
3. Assay medium: serum free RPMI 1640
4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)
5. Incubation time : 24 hr (after sample treatment)
6. Concentration range: 10pg/ml - 10ug/ml
7. Detection method : BrdU assay
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 21.6 kDa (207 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
Alternate Names for VEGF-B Recombinant Protein
- vascular endothelial growth factor B
- VEGF-related factor
VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1(VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis. Recombinant VEGF-B protein was expressed in E.coli and purified by using conventional chromatography techniques.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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