Recombinant Human VEGF-B Protein

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SDS-Page: Recombinant Human VEGF-B Protein [NBC1-22606] - 15% SDS-PAGE (3 ug).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBC1-22606
    • Availability
      Product Discontinued

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Recombinant Human VEGF-B Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 22-207 of Human VEGF-B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA

Details of Functionality
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
VEGFB
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human VEGF-B Protein

  • vascular endothelial growth factor B
  • VEGFB
  • VEGF-B
  • VEGF-related factor
  • VRFVEGFL

Background

VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1(VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis. Recombinant VEGF-B protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Contact Information

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Bioinformatics

Gene Symbol VEGFB
Entrez