Western Blot: VEGF Antibody (5C3.F8) [NB600-548] - Analysis of 1ug of indicated recombinant VEGF isoforms, and 10ul of PageRuler PlusPrestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel.
Immunocytochemistry/ Immunofluorescence: VEGF Antibody (5C3.F8) [NB600-548] - Analysis of Vascular Endothelial Growth Factor (VEGF, green) in Hela cells. The cells were fixed with 4% paraformaldehyde, permeabilized with ...read more
Western Blot: VEGF Antibody (5C3.F8) [NB600-548] - Analysis of VEGF from Human Cells in a reducing gel.
This antibody can be used in Immunohistochemistry-FFPE and Western Blot. By Western blot, this antibody detects a band of 19-22 kDa (reduced), which corresponds to the predicted molecular weight of VEGF. Not tested in other applications. Optimal dilutions should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Alternate Names for VEGF Antibody (5C3.F8)
vascular endothelial growth factor A
Vascular permeability factor
VPFvascular endothelial growth factor
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for VEGF Antibody (NB600-548). (Showing 1 - 1 of 1 FAQs).
Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
VEGF: Vascular endothelial growth factor VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g... Read full blog post.
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