Recombinant Human VEGF 121 Protein

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Functional: VEGF 121 Protein [NBC1-18520] - NIH/3T3 cell proliferation was determined by BrdU incorporation method at 405 nm. This test was performed duplicate. N.C. - negative control [only 0.1% FBS] P.C. - positive ...read more
SDS-Page: VEGF 121 Protein [NBC1-18520] - VEGF121, 16.3 kDa (142aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human VEGF 121 Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 207-327 of Human VEGF 121

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR

Details of Functionality
The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
VEGFA
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
16.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris pH 8.0 containing 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human VEGF 121 Protein

  • MVCD1
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • VEGF-A
  • VEGFMGC70609
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol VEGFA