VEGF 121 Recombinant Protein Summary
A recombinant protein with a His-tag corresponding to amino acids 207 to 327 of Human VEGF 121
Source: E. coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
|Details of Functionality
The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Liquid in 20mM Tris pH 8.0 containing 10% glycerol
Cell line: NIH-3T3 (Mus mosculus/ embryo)
Maintenance Condition: RPMI 1640 containing 10% FBS
Assay Medium: serum free RPMI 1640
Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul)
Serum Free Starvation: 24hr with RPMI1640
Incubation Time: 24 hr (after sample treatment)
Concentration Range: 100 pg/ml - 10 ug/ml
Detection method: BrdU assay
The purity of this protein is > 95% by SDS PAGE. Molecular weight is 16.3 kDa (142aa), confirmed by MALDI-TOF.
Alternate Names for VEGF 121 Recombinant Protein
- vascular endothelial growth factor A
- Vascular permeability factor
- VPFvascular endothelial growth factor
Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E.coli and purified by using conventional chromatography techniques.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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