VECTASTAIN® ABC-AMP Reagent

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Western Blot: VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB] - Serial dilutions (1:2) of maltose binding protein (MBP) were resolved by electrophoresis on a 12% PAGE reducing gel, transferred onto nitrocellulose membrane ...read more
VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB]

Product Details

Summary
Applications WB, ESPOT

Order Details

VECTASTAIN® ABC-AMP Reagent Summary

Description
Features:

  • Amplified ABC alkaline phosphatase reagent for protein blot detection
  • Uses biotin/streptavidin technology
  • Use with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate to produce a very high and sustained light emissions signal, with low background, and permanent fluorescence
  • Can detect as little as 1 pg target protein when used with DuoLuX™ Substrate
  • Applications/Dilutions

    Application Notes
    The VECTASTAIN ABC-AmP Reagent is sufficient to develop approximately twenty 100 cm2 blots.

    Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.

    Kit Components

    Components
    1. 0.5 ml Reagent A
    2. 0.5 ml Reagent B

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

    The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

    The sensitivity of VECTASTAIN ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

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    Video Protocols

    WB Video Protocol

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