VECTASTAIN® ABC-AMP Reagent

Western Blot: VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB] - Serial dilutions (1:2) of maltose binding protein (MBP) were resolved by electrophoresis on a 12% PAGE reducing gel, transferred onto nitrocellulose membrane and detected with biotinylated antibody.

VECTASTAIN(R) ABC-AMP Reagent [AK-6000-NB]

• Applications: WB, ESPOT

VECTASTAIN® ABC-AMP Reagent Summary

• Description: Features:
  
  
• Amplified ABC alkaline phosphatase reagent for protein blot detection
• Uses biotin/streptavidin technology
• Use with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate to produce a very high and sustained light emissions signal, with low background, and permanent fluorescence
• Can detect as little as 1 pg target protein when used with DuoLuX™ Substrate

Applications/Dilutions

• Dilutions:
  • ELISPOT
  • Western Blot
• Application Notes: The VECTASTAIN ABC-AmP Reagent is sufficient to develop approximately twenty 100 cm2 blots.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

The sensitivity of VECTASTAIN ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.