VECTASTAIN® ABC-AMP Reagent Summary
Description |
Features:
Amplified ABC alkaline phosphatase reagent for protein blot detection
Uses biotin/streptavidin technology
Use with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate to produce a very high and sustained light emissions signal, with low background, and permanent fluorescence
Can detect as little as 1 pg target protein when used with DuoLuX™ Substrate |
Applications/Dilutions
Dilutions |
|
Application Notes |
The VECTASTAIN ABC-AmP Reagent is sufficient to develop approximately twenty 100 cm2 blots. |
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Kit Components
Components
|
- 0.5 ml Reagent A
- 0.5 ml Reagent B
|
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Background
Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.
The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.
The sensitivity of VECTASTAIN ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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