VASP Antibody (4D6)

Images

 
Western Blot: VASP Antibody (4D6) [NBP2-00555] Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: VASP Antibody (4D6) [NBP2-00555] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.
Immunohistochemistry-Paraffin: VASP Antibody (4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymphoma tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (4D6) [NBP2-00555] - Analysis of Jurkat cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: VASP Antibody (4D6) [NBP2-00555] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: VASP Antibody (4D6) [NBP2-00555] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (4D6) [NBP2-00555] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (4D6) [NBP2-00555] - Staining of paraffin-embedded Human endometrium tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (4D6) [NBP2-00555] - Staining of paraffin-embedded Human liver tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: VASP Antibody (4D6) [NBP2-00555] - Staining of paraffin-embedded Human lymph node tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (4D6) [NBP2-00555] - Analysis of Hela cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
4D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

VASP Antibody (4D6) Summary

Immunogen
Full length human recombinant protein of human VASP(NP_003361) produced in 293T Cell.
Marker
Focal Adhesion Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VASP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VASP Antibody (4D6)

  • vasodilator-stimulated phosphoprotein
  • VASP

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VASP
Entrez